製品の概要

  • 製品名Anti-NACA2 antibody
  • 製品の詳細
    Mouse polyclonal to NACA2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human NACA2 (NP_954984.1).

  • ポジティブ・コントロール
    • NACA2 transfected 293T lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 24 kDa).

ターゲット情報

  • 関連性NACA2, Nascent polypeptide associated complex subunit alpha 2, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER) by binding to nascent polypeptide chains as they emerge from the ribosome and blocking their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. NACA2 also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites).
  • 細胞内局在Cytoplasmic and Nuclear
  • 参照データベース
  • 別名
    • Alpha NAC like antibody
    • ANAC antibody
    • Hom s 2.01 antibody
    • MGC71999 antibody
    • NAC alpha like antibody
    • NACA2 antibody
    • NACAL antibody
    • nascent polypeptide associated complex alpha polypeptide like antibody
    • Nascent polypeptide associated complex subunit alpha 2 antibody
    see all

Anti-NACA2 antibody 画像

  • All lanes : Anti-NACA2 antibody (ab69439) at 1/500 dilution

    Lane 1 : NACA2 transfected 293T lysate
    Lane 2 : Non transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 24 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

Anti-NACA2 antibody (ab69439) 使用論文

ab69439 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Embryonic fibroblast)
Gel Running Conditions Reduced Denaturing (4-20% gradient gel)
Loading amount 30 µg
Specification Embryonic fibroblast
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

YanPing Zhu

Verified customer

投稿 Jul 06 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"