製品の概要

  • 製品名Anti-NACA1 antibody
  • 製品の詳細
    Mouse polyclonal to NACA1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length NACA1 protein (Human)

  • ポジティブ・コントロール
    • NACA1 transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab67872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 23 kDa).

ターゲット情報

  • 機能Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.
  • 組織特異性Ubiquitously expressed.
  • 配列類似性Belongs to the NAC-alpha family.
    Contains 1 NAC-A/B (NAC-alpha/beta) domain.
    Contains 1 UBA domain.
  • 翻訳後修飾Phosphorylation of Ser-43 by ILK during cell adhesion may promote nuclear localization. Phosphorylation of Thr-159 by GSK3B may promote proteasome mediated degradation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm. Nucleus. Predominantly cytoplasmic. Also found in nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha NAC antibody
    • Alpha-NAC antibody
    • Hom s 2.02 antibody
    • HSD48 antibody
    • MGC117224 antibody
    • NAC-alpha antibody
    • naca antibody
    • NACA/1.9.2 antibody
    • NACA_HUMAN antibody
    • NACA1 antibody
    • Nascent polypeptide associated complex alpha polypeptide antibody
    • Nascent polypeptide associated complex alpha subunit antibody
    • Nascent polypeptide associated complex subunit alpha antibody
    • Nascent polypeptide-associated complex subunit alpha antibody
    see all

Anti-NACA1 antibody 画像

  • All lanes : Anti-NACA1 antibody (ab67872) at 1/500 dilution

    Lane 1 : NACA1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 23 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

Anti-NACA1 antibody (ab67872) 使用論文

ab67872 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate)
Specification Prostate
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novacastra, pH 6.0
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 22°C
Username

Dr. Aamir Ahmed

Verified customer

投稿 Sep 07 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"