製品の概要

  • 製品名Anti-Nab2 antibody
    Nab2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nab2
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee
  • 免疫原

    A region within synthetic peptide: MHRAPSPTAE QPPGGGDSAR RTLQPRLKPS ARAMALPRTL GELQLYRVLQ, corresponding to N terminal amino acids 1-50 of Human Nab2

  • ポジティブ・コントロール
    • HepG2 cells

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab66604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

ターゲット情報

  • 機能Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.
  • 組織特異性Widely expressed at low levels. Highly expressed in melanoma cell lines.
  • 配列類似性Belongs to the NAB family.
  • ドメインThe NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
    The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
  • 細胞内局在Nucleus. Isoform 2 is not localized to the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EGR 1 binding protein 2 antibody
    • EGR-1-binding protein 2 antibody
    • EGR1 binding protein 2 antibody
    • MADER antibody
    • Melanoma associated delayed early response protein antibody
    • Melanoma-associated delayed early response protein antibody
    • MGC75085 antibody
    • Nab 2 antibody
    • nab2 antibody
    • NAB2_HUMAN antibody
    • NGFI A binding protein 2 (EGR1 binding protein 2) antibody
    • NGFI A binding protein 2 antibody
    • NGFI-A-binding protein 2 antibody
    • Protein MADER antibody
    see all

Anti-Nab2 antibody 画像

  • All lanes : Anti-Nab2 antibody (ab66604) at 1/1.25 dilution (in 5% skim milk / PBS buffer)

    Lane 1 : marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 48,62 kDa (why is the actual band size different from the predicted?)

Anti-Nab2 antibody (ab66604) 使用論文

ab66604 has not yet been referenced specifically in any publications.

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