製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Nab2
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 475-525 of Human Nab2 (NP_005958.1)

  • ポジティブ・コントロール
    • Whole cell lysate from 293T and Jurkat cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab112056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 57 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 機能
    Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.
  • 組織特異性
    Widely expressed at low levels. Highly expressed in melanoma cell lines.
  • 配列類似性
    Belongs to the NAB family.
  • ドメイン
    The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
    The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
  • 細胞内局在
    Nucleus. Isoform 2 is not localized to the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EGR 1 binding protein 2 antibody
    • EGR-1-binding protein 2 antibody
    • EGR1 binding protein 2 antibody
    • MADER antibody
    • Melanoma associated delayed early response protein antibody
    • Melanoma-associated delayed early response protein antibody
    • MGC75085 antibody
    • Nab 2 antibody
    • nab2 antibody
    • NAB2_HUMAN antibody
    • NGFI A binding protein 2 (EGR1 binding protein 2) antibody
    • NGFI A binding protein 2 antibody
    • NGFI-A-binding protein 2 antibody
    • Protein MADER antibody
    see all

画像

  • All lanes : Anti-Nab2 antibody (ab112056) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg/ml
    Lane 2 : HeLa whole cell lysate at 15 µg/ml
    Lane 3 : HeLa whole cell lysate at 5 µg/ml
    Lane 4 : Jurkat whole cell lysate at 50 µg/ml


    Predicted band size : 57 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation of NAB2. Samples: 1 mg of whole cell lysate from 293T cells. NAB2 was detected with anti-NAB2 antibody NAB2 antibody (ab112056) at 1 µg/ml. Lane 1- NAB2 was also immunoprecipitated by rabbit anti-NAB2 antibody NAB2 antibody (ab112056) at 6 µg/mg lysate. Lane 2- NAB2 was immunoprecipitated by an rabbit anti-NAB2 antibody that recognizes an upstream epitope. Lane 3- Rabbit IgG control. Detection: Chemiluminescence with exposure time of 10 seconds.

参考文献

ab112056 has not yet been referenced specifically in any publications.

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