Application
Western blot
Sample
Mouse Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s)
Dilution
1/200
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Donkey anti-goat HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Clarity
Exposure
8 minute(s) and 0 second(s)
Bands
Specific: 0 kDa Non-specific: 22, 50, 57 kDa
Additional data
Additional Notes
Lane 1: ladder
Lane 2: mouse intestinal tissue lysate
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The protein nNOS is detected in the rat intestine (https://www.abcam.com/nNOS-neuronal-antibody-ab1376/reviews/55343). Its expression is thus expected in mouse intestine.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 11 2016