製品の概要

  • 製品名Anti-N4BP1 antibody
    N4BP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to N4BP1
  • 特異性This antibody detects endogenous levels of total N4BP1 protein.
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthesized peptide derived from an internal region of human N4BP1.

  • ポジティブ・コントロール
    • Extracts from MCF-7 cells, 293 cells and Jurkat cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 24831002
WB 1/500 - 1/1000. Predicted molecular weight: 100 kDa.
ELISA 1/40000.

ターゲット情報

  • 機能Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates.
  • 組織特異性Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.
  • 配列類似性Belongs to the N4BP1 family.
  • 翻訳後修飾Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated by SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus > nucleolus. Nucleus > PML body. Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ31821 antibody
    • KIAA0615 antibody
    • MGC176730 antibody
    • N4BP1 antibody
    • N4BP1_HUMAN antibody
    • NEDD4 binding protein 1 antibody
    • NEDD4-binding protein 1 antibody
    see all

Anti-N4BP1 antibody 画像

  • All lanes : Anti-N4BP1 antibody (ab74260) at 1/500 dilution

    Lane 1 : Extracts from MCF-7 cells
    Lane 2 : Extracts from 293 cells
    Lane 3 : Extracts from Jurkat cells
    Lane 4 : Extracts from MCF-7 cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 100 kDa
    Observed band size : 100 kDa

Anti-N4BP1 antibody (ab74260) 使用論文

This product has been referenced in:
  • Sugeno N  et al. Lys-63-linked Ubiquitination by E3 Ubiquitin Ligase Nedd4-1 Facilitates Endosomal Sequestration of Internalized a-Synuclein. J Biol Chem 289:18137-18151 (2014). WB, ICC/IF . Read more (PubMed: 24831002) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways: (1) Redeemed aga...

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Thank you for contacting Abcam regarding ab74260. I am sorry that you are experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and would like to make some suggestions to improve your results with...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"