製品の概要

  • 製品名Anti-N4BP1 antibody
    N4BP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to N4BP1
  • 特異性At least three isoforms of N4BP1 are known to exist; ab106649 will only detect the longest isoform.
  • アプリケーション適用あり: ICC, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    An 18 amino acid synthetic peptide near the N terminus of Human N4BP1 (NP_694574).

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at 4°C (stable for up to 12 months).
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab106649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 100 kDa.

ターゲット情報

  • 機能Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates.
  • 組織特異性Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.
  • 配列類似性Belongs to the N4BP1 family.
  • 翻訳後修飾Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated by SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus > nucleolus. Nucleus > PML body. Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ31821 antibody
    • KIAA0615 antibody
    • MGC176730 antibody
    • N4BP1 antibody
    • N4BP1_HUMAN antibody
    • NEDD4 binding protein 1 antibody
    • NEDD4-binding protein 1 antibody
    see all

Anti-N4BP1 antibody 画像

  • Immunofluorescence of N4BP1 in HeLa cells with ab106649 at 20 ug/mL.
  • All lanes : Anti-N4BP1 antibody (ab106649) at 0.5 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 100 kDa
  • Immunocytochemistry of N4BP1 in HeLa cells with ab106649 at 10 ug/mL.

Anti-N4BP1 antibody (ab106649) 使用論文

ab106649 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"