製品の概要

  • 製品名Anti-N myc interactor antibody
    N myc interactor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to N myc interactor
  • 特異性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: IHC-P, WBmore details
    適用なし: IP
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between C terminal residues 250 and 307 of human N myc interactor. (NP_004679.1)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 関連性NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT mediated transcription in response to cytokines IL2 and IFN gamma. The NMI mRNA has high expression in myeloid leukemia cell lines.
    • 細胞内局在Cytoplasmic
    • 参照データベース
    • 別名
      • N myc (and STAT) interactor antibody
      • N myc and STAT interactor antibody
      • N myc interactor antibody
      • NMI antibody
      • NMYC and STAT interactor antibody
      • NMYC interactor antibody
      see all

    Anti-N myc interactor antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and bladder carcinoma (right) tissues labelling N myc interactor with ab70210 at 1/1000 (0.2 µg/ml). Detection: DAB.
    • All lanes : Anti-N myc interactor antibody (ab70210) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 5 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 50
      Lane 4 : Whole cell lysate from 293T cells at 50
      Lane 5 : Whole cell lysate from NIH3T3 cells at 50


      Predicted band size : 35 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

    Anti-N myc interactor antibody (ab70210) 使用論文

    ab70210 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"