Anti-n-Myc 抗体 [NCM II 100] - ChIP Grade (ab16898)

製品の概要

  • 製品名Anti-n-Myc antibody [NCM II 100] - ChIP Grade
    n-Myc 一次抗体 製品一覧
  • 製品の詳細
    Mouse Monoclonal [NCM II 100] to n-Myc - ChIP Grade
  • 特異性This antibody reacts with N-myc encoded proteins and their cleavage products.
  • アプリケーション適用あり: IHC-Fr, IP, WB, ICC/IF, Flow Cyt, ChIPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Fusion protein corresponding to Human n-Myc. Recombinant fusion protein. Original clone reference: PubMed ID - 2426708.
    Database link: P04198

  • ポジティブ・コントロール
    • IMR5 Cells
  • 特記事項It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use a concentration of 1 - 5 µg/ml.

See references

IP Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64, 67 kDa).

See references

ICC/IF Use a concentration of 1 - 5 µg/ml.

Pubmed ID: 2426708

Flow Cyt Use 0.5µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ChIP Use at an assay dependent concentration. PubMed: 19495417Suggested to be used at 2µg.

ターゲット情報

  • 機能May function as a transcription factor.
  • 関連疾患Note=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
    Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
    Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585].
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 発生段階Expressed during fetal development.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bHLHe37 antibody
    • Class E basic helix-loop-helix protein 37 antibody
    • MODED antibody
    • mycn antibody
    • MYCN_HUMAN antibody
    • N myc antibody
    • N myc proto oncogene protein antibody
    • N-myc proto-oncogene protein antibody
    • Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene antibody
    • Neuroblastoma MYC oncogene antibody
    • NMYC antibody
    • NMYC proto oncogene protein antibody
    • ODED antibody
    • Oncogene NMYC antibody
    • pp65/67 antibody
    • v myc avian myelocytomatosis viral related oncogene neuroblastoma derived antibody
    • v myc myelocytomatosis viral related oncogene neuroblastoma derived antibody
    see all

Anti-n-Myc antibody [NCM II 100] - ChIP Grade 画像

  • Immunoprecipitation of N-Myc from S35 methionine-labeled IMR-5 cells using ab16898 (lane 1) and Normal Mouse IgG (lane 2).
    ab16898 used at 1 mg/sample.
  • Overlay histogram showing SH-SY5Y cells stained with ab16898 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16898, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-n-Myc antibody [NCM II 100] - ChIP Grade (ab16898) 使用論文

This product has been referenced in:

See all 16 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (NuPage Bis-Tris 4-12% gel)
Sample Mouse Cell lysate - whole cell (cerebellar granule neuron precursors)
Specification cerebellar granule neuron precursors
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Nov 26 2013


What species is your lysate from?


For human samples, the n-myc oncoprotein has a predicted MW of 50 kDa (http://www.uniprot.org/uniprot/P04198), but runs in WB at around 64 kDa. Cleavage products would run at smaller MWs.

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Neural tube)
Specification Neural tube
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate/Citrate+NaBH4/Tris-EDTA (ph=9)/10% SDS/ NaBH4/10% SDS + NaBH4
Permeabilization Yes - 0.5% Triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
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Abcam user community

Verified customer

投稿 Mar 19 2010

Thank you for your enquiry. According to the following publication, monoclonal ab16898 n-Myc antibody [clone NCM II 100] interacts with an epitope unique to an N-myc region not present in C-myc regions tested (3' 60% mouse C-myc coding region and 5'...

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Thank you for your enquiry. This antibody has been characterised with others raised against NMYC in the following paper which may be of help to you: Identification and characterisation of the NMYC gene product in human neuroblastoma cells by monocl...

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Thank you for contacting us for technical support. The problem you are experiencing may be due to the fact that the antibody is very fragile. We recommend to aliquot and store at -20°C upon arrival. Can you tell me how you proceeded? If you can also t...

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Thank you for your researcher's further information. The information that accompanies this antibody is that the buffer is comprised of no preservatives as detailed below: Preservative: None Constituents: 50% Glycerol, 0.05M Sodium phosphate. Furt...

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We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to go ahead and purc...

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Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC-formalin fixed paraffin embedded sections. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, p...

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