製品の概要

  • 製品名
    Anti-n-Myc antibody [6A4I]
    n-Myc 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [6A4I] to n-Myc
  • アプリケーション
    適用あり: WB, ChIP, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human n-Myc.
    Database link: P04198

  • ポジティブ・コントロール
    • HeLa cell, Ramos cell, HuT78 cell, MCF7 cell, Mouse Brain tissue, Mouse Testis tissue, Rat Brain tissue and Rat Testis tissue lysates.
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR267858 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
ChIP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    May function as a transcription factor.
  • 関連疾患
    Note=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
    Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
    Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585].
  • 配列類似性
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • 発生段階
    Expressed during fetal development.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bHLHe37 antibody
    • Class E basic helix-loop-helix protein 37 antibody
    • MODED antibody
    • mycn antibody
    • MYCN_HUMAN antibody
    • N myc antibody
    • N myc proto oncogene protein antibody
    • N-myc proto-oncogene protein antibody
    • Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene antibody
    • Neuroblastoma MYC oncogene antibody
    • NMYC antibody
    • NMYC proto oncogene protein antibody
    • ODED antibody
    • Oncogene NMYC antibody
    • pp65/67 antibody
    • V myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog antibody
    • v myc avian myelocytomatosis viral related oncogene neuroblastoma derived antibody
    • v myc myelocytomatosis viral related oncogene neuroblastoma derived antibody
    see all

Anti-n-Myc antibody [6A4I] 画像

  • Overlay histogram showing SH-SH5Y cells stained with ab119701 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119701, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • All lanes : Anti-n-Myc antibody [6A4I] (ab119701) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Ramos cell lysate
    Lane 3 : HuT78 cell lysate
    Lane 4 : MCF7 cell lysate
    Lane 5 : Mouse Brain tissue lysate
    Lane 6 : Mouse Testis tissue lysate
    Lane 7 : Rat Brain tissue lysate
    Lane 8 : Rat Testis tissue lysate


    Predicted band size : 50 kDa

Anti-n-Myc antibody [6A4I] (ab119701) 使用論文

ab119701 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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