Anti-N Cadherin 抗体 [EPR1791-4] (ab76011)

製品の概要

  • 製品名Anti-N Cadherin antibody [EPR1791-4]
    N Cadherin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1791-4] to N Cadherin
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt or ICC/IF
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in the extracellular region of human N Cadherin.

  • ポジティブ・コントロール
    • Huvec, C6 and HeLa cell lysates.
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/20000. Predicted molecular weight: 100 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for Flow Cyt or ICC/IF.
  • ターゲット情報

    • 機能Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
    • 配列類似性Contains 5 cadherin domains.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CADH2_HUMAN antibody
      • Cadherin 2 antibody
      • Cadherin 2 N cadherin neuronal antibody
      • Cadherin 2 type 1 antibody
      • Cadherin 2 type 1 N cadherin neuronal antibody
      • Cadherin 2, type 1, N-cadherin (neuronal) antibody
      • Cadherin-2 antibody
      • Cadherin2 antibody
      • Calcium dependent adhesion protein neuronal antibody
      • CD325 antibody
      • CD325 antigen antibody
      • CDH2 antibody
      • CDHN antibody
      • CDw325 antibody
      • CDw325 antigen antibody
      • N cadherin 1 antibody
      • N-cadherin antibody
      • NCAD antibody
      • Neural cadherin antibody
      • OTTHUMP00000066304 antibody
      • OTTHUMP00000067378 antibody
      see all

    Anti-N Cadherin antibody [EPR1791-4] 画像

    • ab76011 staining N Cadherin in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA + 1% FBS for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer pH6. Samples were incubated with primary antibody (1/500 in 1% BSA + 1% FBS) for 16 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Lanes 1 - 2 : Anti-N Cadherin antibody [EPR1791-4] (ab76011) at 1/20000 dilution
      Lane 3 : Anti-N Cadherin antibody [EPR1791-4] (ab76011) at 1/5000 dilution

      Lane 1 : Huvec cell lysate
      Lane 2 : C6 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 100 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)

    Anti-N Cadherin antibody [EPR1791-4] (ab76011) 使用論文

    This product has been referenced in:
    • Hwang PY  et al. N-cadherin is Key to Expression of the Nucleus Pulposus Cell Phenotype under Selective Substrate Culture Conditions. Sci Rep 6:28038 (2016). IHC-P . Read more (PubMed: 27292569) »
    • Hu CT  et al. MIF, secreted by human hepatic sinusoidal endothelial cells, promotes chemotaxis and outgrowth of colorectal cancer in liver prometastasis. Oncotarget 6:22410-23 (2015). ICC/IF ; Human . Read more (PubMed: 26087187) »

    See all 9 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Cow Tissue sections (oviductal tissues)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9.0
    Permeabilization No
    Specification oviductal tissues
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    芳彦 小林

    Verified customer

    投稿 Sep 21 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (MCF-7 cell)
    Gel Running Conditions Reduced Denaturing (10%)
    Loading amount 30 µg
    Specification MCF-7 cell
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 11 2016

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Embryonic mouse brain coronal cortical section)
    Permeabilization Yes - Triton X 100
    Specification Embryonic mouse brain coronal cortical section
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Dr. Bhavin Shah

    Verified customer

    投稿 Oct 20 2015

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (10)
    Sample Mouse Cell lysate - whole cell (MEFs)
    Specification MEFs
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Dec 16 2013

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step 1%BSA 1%FBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
    Sample Mouse Tissue sections (Pancreas)
    Specification Pancreas
    Permeabilization Yes - TBS-Tween 0,1%
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jun 06 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (MEFs wild type)
    Specification MEFs wild type
    Fixative Paraformaldehyde
    Permeabilization Yes - 0,3% Triton PBS
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 18 2013

    Thank you for contacting us and your interest in our products.
    The anti-N Cadherin antibody [8C11], ab19348, was raised against a bacterial fusion protein corresponding to the extracellular domain of N-cadherin (a domain between EC3 and EC4, amino...

    Read More

    Thank you for your reply.

    Can you please confirm what species your samples are from, including cell line or tissue type?

    Also what is the order number for this product? I will need to ensure that the order is not more than 6 months ...

    Read More

    Thank you for your reply.

    You're having trouble with the ab76011, anti-N cadherin antibody, correct? Not the ab1791 histone H3? You had included ab76011 in your initial email, but ab1791 in your reply.

    Do you have a lot # from the via...

    Read More

    Thank you very much for your interest in Abcam antibodies.

    We have 2 rabbitantibodies directed against the extracellular part of the N-cadherin :

    http://www.abcam.com/ab76011, rabbit monoclonal, tested in Western Blot, ICC/IF, Flow ...

    Read More

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