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Unphosphorylated synthetic peptide:
ERPIHAEPQYPVRS, corresponding to amino acids 811-824 of Human N Cadherin (coupled to a carrier protein). This sequence is conserved in rat and mouse N cadherin, and has three amino acid differences from the conserved region in R Cadherin.
Our Abpromise guarantee covers the use of ab76057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 100 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Western blot image of mouse brain lysate immunoprecipitated with no antibody (lane 1), anti-N-Cadherin (CP1751) rabbit polyclonal antibody (lane 2), and whole mouse brain lysate (lane 3). The blot was probed with anti-N-cadherin (Cytoplasmic) mouse monoclonal antibody (lanes 1-3) and detected using anti-Mouse Ig Light Chain specific:HRP secondary antibody.
This image is courtesy of an anonymous Abreview
IHC image of N Cadherin staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76057, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab76057 staining N Cadherin in human BicR19 cells by Flow Cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100) for 30 minutes. An undiluted Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Gating Strategy: Oral epithelial cells.