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Our Abpromise guarantee covers the use of ab12221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 - 10 µg/ml. Use under non reducing condition. Predicted molecular weight: 99.9 kDa.|
|IHC-Fr||Use a concentration of 5 - 10 µg/ml.|
|IHC-P||Use a concentration of 5 - 10 µg/ml.|
Image courtesy of an anonymous Abreview.Distinct bands were found in all tested cell lines with no unspecific bands.
ab12221 at 1/200 dilution staining rat heart tissue sections by IHC-P. The tissue was fixed with Histochoice and paraffin sectioned prior to staining. An Alexa-Fluor conjugated donkey anti-rabbit antibody was used as the secondary.
The image depicts stained N-Cadherin (green) and counterstained nuclei (DAPI-blue) overlay (left panel). The DIC (phase) image of the same tissue, superimposed with fluorescence overlay (right panel) confirms the localization of the N-Cadherin at intercalated discs region. Note, the phase contrast image shows typical uninucleated, cylindrical morphology of cardiac myocytes; that are often branched and joined to each other end-to-end (intercalated discs) to form interlacing network.
This image is courtesy of an anonymous Abreview