製品の概要

  • 製品名Anti-N Cadherin antibody [8C11]
    N Cadherin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [8C11] to N Cadherin
  • 特異性This antibody is not suitable for mouse C2C12 cell lysates in Western blot analysis.
  • アプリケーション適用あり: WB, ICC/IF, IPmore details
  • 種交差性
    交差種: Rabbit, Hamster, Human, Bird
    非交差種: Mouse, Rat, Cow, Pig
  • 免疫原

    Fusion protein corresponding to N Cadherin (extracellular).

  • ポジティブ・コントロール
    • A431 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 100 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • 配列類似性Contains 5 cadherin domains.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CADH2_HUMAN antibody
    • Cadherin 2 antibody
    • Cadherin 2 N cadherin neuronal antibody
    • Cadherin 2 type 1 antibody
    • Cadherin 2 type 1 N cadherin neuronal antibody
    • Cadherin 2, type 1, N-cadherin (neuronal) antibody
    • Cadherin-2 antibody
    • Cadherin2 antibody
    • Calcium dependent adhesion protein neuronal antibody
    • CD325 antibody
    • CD325 antigen antibody
    • CDH2 antibody
    • CDHN antibody
    • CDw325 antibody
    • CDw325 antigen antibody
    • N cadherin 1 antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural cadherin antibody
    • OTTHUMP00000066304 antibody
    • OTTHUMP00000067378 antibody
    see all

Anti-N Cadherin antibody [8C11] 画像

  • Lane 1 : Anti-N Cadherin antibody [8C11] (ab19348) at 1/1000 dilution
    Lanes 2 - 3 : Anti-N Cadherin antibody [8C11] (ab19348) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HUVEC cell lysate
    Lane 3 : C2C12 cell lysate

    Lysates/proteins at 25 µg per lane.


    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of ARPE-19 cells, staining N Cadherin (green) with ab19348, at 1/20 dilution.

    ARPE-19 monolayer cultures were fixed in paraformaldehyde, permeabilized with 0.2% Triton X-100 for 15 min and blocked with 2% BSA for 30 min. Samples were incubated with primary antibody for 16 hours at 4°C before incubation with an AlexaFluor®488-conjugated donkey anti-mouse secondary IgG for 60 min.

Anti-N Cadherin antibody [8C11] (ab19348) 使用論文

This product has been referenced in:
  • Chen X  et al. S100 calcium-binding protein A6 promotes epithelial-mesenchymal transition through ß-catenin in pancreatic cancer cell line. PLoS One 10:e0121319 (2015). WB ; Human . Read more (PubMed: 25799022) »
  • Zhu YT  et al. Activation of RhoA-ROCK-BMP signaling reprograms adult human corneal endothelial cells. J Cell Biol 206:799-811 (2014). Human . Read more (PubMed: 25202030) »

See all 8 Publications for this product

Product Wall

Thank you for contacting us and your interest in our products.
The anti-N Cadherin antibody [8C11], ab19348, was raised against a bacterial fusion protein corresponding to the extracellular domain of N-cadherin (a domain between EC3 and EC4, amino...

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Thank you for your inquiry.

The immunogen of ab66025 is the full length protein and to our knowledge this clone was not mapped. We therefore do not know where exactly this antibody binds to within the N Cadherin.

According to Swis...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Tissue lysate - whole (Eye)
Loading amount 20 µg
Specification Eye
Gel Running Conditions Reduced Denaturing (8% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

投稿 Jul 07 2009

Thank you for your enquiry. Here is the link to the Clustal W alignment and I will also paste the alignment below. http://www.ebi.ac.uk/cgi-bin/clustalw/result?tool=clustalw&jobid=clustalw-20060323-21275762&poll=yes The immunogen of ab19348 wa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"