製品の概要

  • 製品名
    N-Arachidonylglycine (NAGly)
  • 製品の詳細
    T-type Ca2+ channel blocker. Endogenous GLYT2 inhibitor.
  • 別名
    • NAGly
  • 生理活性の詳細
    Endogenous inhibitor of GLYT2 with little or no effect on GLYT1 and is analgesic in rat models of neuropathic and inflammatory pain. Anandamide-like compound, with little affinity at CB1 receptors, VR1 receptors and anandamide transporters. Potent inhibitor of T-type Ca2+ channels with little activity on anandamide-sensitive TRPV1 and TASK1 currents and high-voltage-activated (HVA) Cav1.2, Cav2.2, Nav1.7, and Nav1.8 currents.
  • 精製度
    > 98%

製品の特性

画像

  • ab32557 staining p38 (phospho T180 + Y182) in RAW 264.7 cells treated with N-Arachidonylglycine (ab120346), by ICC/IF. Increase in expression of p38 (phospho T180 + Y182) correlates with increased concentration of N-Arachidonylglycine, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120346 (N-Arachidonylglycine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32557 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

参考文献

This product has been referenced in:
  • Bradshaw HB  et al. Novel endogenous N-acyl glycines identification and characterization. Vitam Horm 81:191-205 (2009). Read more (PubMed: 19647113) »
  • Barbara G  et al. T-type calcium channel inhibition underlies the analgesic effects of the endogenous lipoamino acids. J Neurosci 29:13106-14 (2009). Read more (PubMed: 19846698) »
  • Edington AR  et al. Extracellular loops 2 and 4 of GLYT2 are required for N-arachidonylglycine inhibition of glycine transport. J Biol Chem 284:36424-30 (2009). Read more (PubMed: 19875446) »

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