製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Myt1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Myt1.

    (Peptide available as ab30996.)

  • ポジティブ・コントロール
    • Human Brain Tissue Lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).

ターゲット情報

  • 機能
    Binds to the promoter regions of proteolipid proteins of the central nervous system. May play a role in the development of neurons and oligodendrogalia in the CNS. May regulate a critical transition point in oligodendrocyte lineage development by modulating oligodendrocyte progenitor proliferation relative to terminal differentiation and up-regulation of myelin gene transcription.
  • 組織特異性
    Mostly in developing nervous system. Expressed in neural progenitors and oligodendrocyte lineage cells. More highly expressed in oligodendrocyte progenitors than in differentiated oligodendrocytes.
  • 配列類似性
    Contains 7 C2HC-type zinc fingers.
  • ドメイン
    Contains 7 zinc fingers of the C2HC class arranged in two widely separated clusters. These two domains of DNA binding can function independently and recognize the same DNA sequence.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C20orf36 antibody
    • KIAA0835 antibody
    • KIAA1050 antibody
    • MTF1 antibody
    • Myelin transcription factor 1 antibody
    • Myelin transcription factor I antibody
    • MYT1 antibody
    • MYT1_HUMAN antibody
    • MyTI antibody
    • PLPB1 antibody
    • Proteolipid protein-binding protein antibody
    see all

画像

  • All lanes : Anti-Myt1 antibody (ab30997) at 1 µg/ml

    Lane 1 : Human Liver normal Tissue Lysate
    Lane 2 : Human Brain Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 122 kDa
    Observed band size : 122 kDa
    Additional bands at : 140 kDa (possible post-translational modification).

プロトコール

参考文献

This product has been referenced in:
  • Islam MM  et al. Enhancer Analysis Unveils Genetic Interactions between TLX and SOX2 in Neural Stem Cells and In Vivo Reprogramming. Stem Cell Reports 5:805-15 (2015). EMSA . Read more (PubMed: 26607952) »
  • Yokoyama A  et al. Identification of Myelin Transcription Factor 1 (MyT1) as a Subunit of the Neural Cell Type-specific Lysine-specific Demethylase 1 (LSD1) Complex. J Biol Chem 289:18152-18162 (2014). WB, ChIP . Read more (PubMed: 24828497) »

See all 3 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Username

Dr. Sophie Pezet

Verified customer

投稿 Jul 29 2011

Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (Neuro2a cell)
Total protein in input
10 µg
Specification
Neuro2a cell
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

投稿 Oct 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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