製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MYPN
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 972-1021 (PVPKVYWFKD GKQISKRNEH CKMRREGDGT CSLHIESTTS DDDGNYTIMA) of Human MYPN (NP_115967).

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Constituents: 2% Sucrose, 97% PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab115775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 145 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Component of the sarcomere that tethers together nebulin (skeletal muscle) and nebulette (cardiac muscle) to alpha-actinin, at the Z lines.
  • 組織特異性
    Expressed in adult skeletal muscle and fetal heart.
  • 配列類似性
    Belongs to the myotilin/palladin family.
    Contains 5 Ig-like (immunoglobulin-like) domains.
  • 細胞内局在
    Cytoplasm. Nucleus. Cytoplasm > myofibril > sarcomere. Bound to sarcomere both at the Z-line periphery and in the central I-band region.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 145 kDa sarcomeric protein antibody
    • MYOP antibody
    • Myopalladin antibody
    • MYPN antibody
    • MYPN_HUMAN antibody
    • sarcomeric protein myopalladin antibody
    see all

Anti-MYPN antibody 画像

  • Anti-MYPN antibody (ab115775) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 145 kDa

プロトコール

Anti-MYPN antibody (ab115775) 使用論文

ab115775 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart left ventricle and tibialis anterior muscle)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
50 µg
Specification
Heart left ventricle and tibialis anterior muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 May 10 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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