製品の概要

  • 製品名
    Anti-MYOZ3 antibody
  • 製品の詳細
    Rabbit polyclonal to MYOZ3
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Dog
  • 免疫原

    antigen sequence: CPWQEFVSYR DYQSDGRSHT PSPNDYRNFN KTPVPFGGPL VGGTFPRPGT PFIPEPLSGL ELLRLRPSFN RVAQGWVRNL PESEEL, corresponding to amino acids 166-251 of Human MYOZ3.

  • ポジティブ・コントロール
    • Human skeletal muscle tissue; Human tonsil lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab122540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval at pH6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

ターゲット情報

  • 機能
    Myozenins may serve as intracellular binding proteins involved in linking Z line proteins such as alpha-actinin, gamma-filamin, TCAP/telethonin, LDB3/ZASP and localizing calcineurin signaling to the sarcomere. Plays an important role in the modulation of calcineurin signaling. May play a role in myofibrillogenesis.
  • 組織特異性
    Expressed specifically in skeletal muscle. Not detected in heart.
  • 配列類似性
    Belongs to the myozenin family.
  • 細胞内局在
    Cytoplasm > myofibril > sarcomere > Z line. Localized at the Z-line of skeletal muscle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Calsarcin-3 antibody
    • CS 3 antibody
    • CS3 antibody
    • FATZ-related protein 3 antibody
    • FRP3 antibody
    • MYOZ 3 antibody
    • Myoz3 antibody
    • MYOZ3_HUMAN antibody
    • Myozenin-3 antibody
    see all

画像

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in cytoplasm. Recommended concentration of ab122540 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122540, at 1/350 dilution, staining MYOZ3 in paraffin-embedded Human skeletal muscle tissue by Immunohistochemistry, showing distinct cytoplasmic positivity in myocytes.
  • All lanes : Anti-MYOZ3 antibody (ab122540) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

参考文献

ab122540 has not yet been referenced specifically in any publications.

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