製品の概要

  • 製品名
    Anti-Myotrophin antibody
  • 製品の詳細
    Rabbit polyclonal to Myotrophin
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Dog, Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 36-85 (RKPLHYAADC GQLEILEFLL LKGADINAPD KHHITPLLSA VYEGHVSCVK) of Human Myotrophin (NP_665807).

  • ポジティブ・コントロール
    • Human fetal thymus lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab90918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 13 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • Anti-Myotrophin antibody (ab90918) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal thymus lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 13 kDa

プロトコール

参考文献

This product has been referenced in:
  • Jafarian A  et al. The Generation of Insulin Producing Cells from Human Mesenchymal Stem Cells by MiR-375 and Anti-MiR-9. PLoS One 10:e0128650 (2015). WB . Read more (PubMed: 26047014) »

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Loading amount
200 µg
Gel Running Conditions
Non-reduced Denaturing (15%)
Sample
Dog Cell lysate - whole cell (heart)
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Glen Pyle

Verified customer

投稿 Dec 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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