The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 4 µg/ml.
組織特異性Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
関連疾患Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11. Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
配列類似性Contains 1 IQ domain. Contains 1 myosin head-like domain.
ドメインThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils. Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
細胞内局在Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
Myosin heavy polypeptide 11 smooth muscle antibody
smooth muscle isoform antibody
Smooth muscle myosin heavy chain 11 antibody
Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [SM-M10] 画像
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [SM-M10] (ab81031)
ab81031 at 4µg/ml staining Myosin, smooth muscle heavy chain 1 and 2 by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded human uterus sections), using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of smooth muscles.
Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [SM-M10] (ab81031) 使用論文
This product has been referenced in:
Chmielewski S et al. STAT1-dependent signal integration between IFN? and TLR4 in vascular cells reflect pro-atherogenic responses in human atherosclerosis. PLoS One9:e113318 (2014).
Read more (PubMed: 25478796) »
Sharifpoor S et al. Functional characterization of human coronary artery smooth muscle cells under cyclic mechanical strain in a degradable polyurethane scaffold. Biomaterials32:4816-29 (2011).
Read more (PubMed: 21463894) »