Anti-Myosin Phosphatase (phospho T696) 抗体 (ab59202)

製品の概要

  • 製品名Anti-Myosin Phosphatase (phospho T696) antibody
    Myosin Phosphatase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Myosin Phosphatase (phospho T696)
  • 特異性Detects endogenous levels of Myosin Phosphatase only when phosphorylated at threoine 696.
  • アプリケーション適用あり: IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 696 (RSTPQG)

  • ポジティブ・コントロール
    • Human breast carcinoma tissue

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

ターゲット情報

  • 機能Regulates myosin phosphatase activity.
  • 組織特異性Expressed in striated muscles, specifically in type 2a fibers (at protein level).
  • 配列類似性Contains 6 ANK repeats.
  • 発生段階Induced by 2-fold during pregnancy, including in abdominus rectus muscle.
  • ドメインHeterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
  • 翻訳後修飾Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
    In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
  • 細胞内局在Cytoplasm. Along actomyosin filaments and stress fibers.
  • Information by UniProt
  • 参照データベース
  • 別名
    • M130 antibody
    • MBS antibody
    • Myosin binding subunit antibody
    • Myosin phosphatase target subunit 1 antibody
    • Myosin phosphatase targeting subunit 1 antibody
    • Myosin phosphatase-targeting subunit 1 antibody
    • MYPT1 antibody
    • MYPT1_HUMAN antibody
    • PPP1R12A antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
    • Protein phosphatase 1 regulatory subunit 12A antibody
    • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
    • Protein phosphatase myosin binding subunit antibody
    • Protein phosphatase myosin-binding subunit antibody
    see all

Anti-Myosin Phosphatase (phospho T696) antibody 画像

  • Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab59202 at 1/50 dilution. Samples were untreated (left) or treated with immunizing peptide (right).

Anti-Myosin Phosphatase (phospho T696) antibody (ab59202) 使用論文

ab59202 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

We don't have a polymer kit besides our mouse on mouse polymer kit, ab127055.

http://www.abcam.com/Mouse-on-Mouse-MOM-Polymer-IHC-Kit-ab127055.html

We have ABC kits and EXPOSE kits.

http:/...

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Thank you for your reply.


This is just to go along with the polymer kit that was used. If you are using polymer detection, simply follow the manufacturers instructions. You can omit this step if not using this type of amplification.
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Thank you for contacting Abcam regarding ab59202.



The IHC protocol is as follows:



Deparaffinization

1. Incubate slide at 60℃ for 60 minutes.

2. Deparaffinize in Xylene for 10 minutes and repeat on...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 120 µg
Specification Hela
Treatment 10ng/ml IL6 for 30 minutes
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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投稿 Sep 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"