製品の概要

  • 製品名Anti-Myosin Phosphatase antibody [Y110]
    Myosin Phosphatase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y110] to Myosin Phosphatase
  • 特異性Ab32393 recognises Myosin Phosphatase. It can detect all four splice isoforms of Myosin Phosphatase.
  • アプリケーション適用あり: WB, ICCmore details
    適用なし: Flow Cyt,IHC or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in the first ANK repeat of human Myosin Phosphatase.

  • ポジティブ・コントロール
    • 293 cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 125 kDa (predicted molecular weight: 115 kDa).
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt,IHC or IP.
  • ターゲット情報

    • 機能Regulates myosin phosphatase activity.
    • 組織特異性Expressed in striated muscles, specifically in type 2a fibers (at protein level).
    • 配列類似性Contains 6 ANK repeats.
    • 発生段階Induced by 2-fold during pregnancy, including in abdominus rectus muscle.
    • ドメインHeterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
    • 翻訳後修飾Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
      In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
    • 細胞内局在Cytoplasm. Along actomyosin filaments and stress fibers.
    • Information by UniProt
    • 参照データベース
    • 別名
      • M130 antibody
      • MBS antibody
      • Myosin binding subunit antibody
      • Myosin phosphatase target subunit 1 antibody
      • Myosin phosphatase targeting subunit 1 antibody
      • Myosin phosphatase-targeting subunit 1 antibody
      • MYPT1 antibody
      • MYPT1_HUMAN antibody
      • PPP1R12A antibody
      • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
      • Protein phosphatase 1 regulatory subunit 12A antibody
      • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
      • Protein phosphatase myosin binding subunit antibody
      • Protein phosphatase myosin-binding subunit antibody
      see all

    Anti-Myosin Phosphatase antibody [Y110] 画像

    Anti-Myosin Phosphatase antibody [Y110] (ab32393) 使用論文

    This product has been referenced in:
    • Petrakova OS  et al. Comparative analysis reveals similarities between cultured submandibular salivary gland cells and liver progenitor cells. Springerplus 3:183 (2014). ICC/IF ; Mouse . Read more (PubMed: 24790827) »
    • Kim JH & Adelstein RS LPA(1) -induced migration requires nonmuscle myosin II light chain phosphorylation in breast cancer cells. J Cell Physiol 226:2881-93 (2011). Read more (PubMed: 21302283) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"