リコンビナント
RabMAb

Anti-Myosin Phosphatase 抗体 [Y110] (ab32393)

製品の概要

  • 製品名
    Anti-Myosin Phosphatase antibody [Y110]
    Myosin Phosphatase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y110] to Myosin Phosphatase
  • 特異性
    Ab32393 recognises Myosin Phosphatase. It can detect all four splice isoforms of Myosin Phosphatase.
  • アプリケーション
    適用あり: WB, ICCmore details
    適用なし: Flow Cyt,IHC or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in the first ANK repeat of human Myosin Phosphatase.

  • ポジティブ・コントロール
    • 293 cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 125 kDa (predicted molecular weight: 115 kDa).
ICC 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt,IHC or IP.
  • ターゲット情報

    • 機能
      Regulates myosin phosphatase activity.
    • 組織特異性
      Expressed in striated muscles, specifically in type 2a fibers (at protein level).
    • 配列類似性
      Contains 6 ANK repeats.
    • 発生段階
      Induced by 2-fold during pregnancy, including in abdominus rectus muscle.
    • ドメイン
      Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
    • 翻訳後修飾
      Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
      In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
    • 細胞内局在
      Cytoplasm. Along actomyosin filaments and stress fibers.
    • Information by UniProt
    • 参照データベース
    • 別名
      • M130 antibody
      • MBS antibody
      • Myosin binding subunit antibody
      • Myosin phosphatase target subunit 1 antibody
      • Myosin phosphatase targeting subunit 1 antibody
      • Myosin phosphatase-targeting subunit 1 antibody
      • MYPT1 antibody
      • MYPT1_HUMAN antibody
      • PPP1R12A antibody
      • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
      • Protein phosphatase 1 regulatory subunit 12A antibody
      • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
      • Protein phosphatase myosin binding subunit antibody
      • Protein phosphatase myosin-binding subunit antibody
      see all

    画像

    参考文献

    This product has been referenced in:
    • Petrakova OS  et al. Comparative analysis reveals similarities between cultured submandibular salivary gland cells and liver progenitor cells. Springerplus 3:183 (2014). ICC/IF ; Mouse . Read more (PubMed: 24790827) »
    • Kim JH & Adelstein RS LPA(1) -induced migration requires nonmuscle myosin II light chain phosphorylation in breast cancer cells. J Cell Physiol 226:2881-93 (2011). Read more (PubMed: 21302283) »

    See all 2 Publications for this product

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab32393.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録