Anti-Myosin Phosphatase 抗体 (ab70809)

製品の概要

  • 製品名Anti-Myosin Phosphatase antibody
    Myosin Phosphatase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Myosin Phosphatase
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 800 and the C-terminus (residue 842) of human Myosin Phosphatase (NP_002471.1)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa or 293T cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Regulates myosin phosphatase activity.
  • 組織特異性Expressed in striated muscles, specifically in type 2a fibers (at protein level).
  • 配列類似性Contains 6 ANK repeats.
  • 発生段階Induced by 2-fold during pregnancy, including in abdominus rectus muscle.
  • ドメインHeterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
  • 翻訳後修飾Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
    In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
  • 細胞内局在Cytoplasm. Along actomyosin filaments and stress fibers.
  • Information by UniProt
  • 参照データベース
  • 別名
    • M130 antibody
    • MBS antibody
    • Myosin binding subunit antibody
    • Myosin phosphatase target subunit 1 antibody
    • Myosin phosphatase targeting subunit 1 antibody
    • Myosin phosphatase-targeting subunit 1 antibody
    • MYPT1 antibody
    • MYPT1_HUMAN antibody
    • PPP1R12A antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
    • Protein phosphatase 1 regulatory subunit 12A antibody
    • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
    • Protein phosphatase myosin binding subunit antibody
    • Protein phosphatase myosin-binding subunit antibody
    see all

Anti-Myosin Phosphatase antibody 画像

  • All lanes : Anti-Myosin Phosphatase antibody (ab70809) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 115 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • 1mg whole lysate from HeLa cells was immunoprecipitated using ab70809 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70809 at 1ug/ml. Detection: chemiluminescence with exposure time of 10 seconds.

Anti-Myosin Phosphatase antibody (ab70809) 使用論文

ab70809 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (RT4-D6P2T Rat Schwanoma Cells)
Gel Running Conditions Reduced Denaturing (8%)
Loading amount 20 µg
Specification RT4-D6P2T Rat Schwanoma Cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Lars Riecken

Verified customer

投稿 Nov 06 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"