製品の概要

  • 製品名
    Anti-Myosin Phosphatase antibody
    Myosin Phosphatase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Myosin Phosphatase
  • アプリケーション
    適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human Myosin Phosphatase around the phosphorylation site of threonine 853 (R-S-TP-G-V).

  • ポジティブ・コントロール
    • Human brain tissue and NIH/3T3 cell extracts.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 115 kDa).

ターゲット情報

  • 機能
    Regulates myosin phosphatase activity.
  • 組織特異性
    Expressed in striated muscles, specifically in type 2a fibers (at protein level).
  • 配列類似性
    Contains 6 ANK repeats.
  • 発生段階
    Induced by 2-fold during pregnancy, including in abdominus rectus muscle.
  • ドメイン
    Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
  • 翻訳後修飾
    Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
    In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
  • 細胞内局在
    Cytoplasm. Along actomyosin filaments and stress fibers.
  • Information by UniProt
  • 参照データベース
  • 別名
    • M130 antibody
    • MBS antibody
    • Myosin binding subunit antibody
    • Myosin phosphatase target subunit 1 antibody
    • Myosin phosphatase targeting subunit 1 antibody
    • Myosin phosphatase-targeting subunit 1 antibody
    • MYPT1 antibody
    • MYPT1_HUMAN antibody
    • PPP1R12A antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
    • Protein phosphatase 1 regulatory subunit 12A antibody
    • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
    • Protein phosphatase myosin binding subunit antibody
    • Protein phosphatase myosin-binding subunit antibody
    see all

画像

  • All lanes : Anti-Myosin Phosphatase antibody (ab59235) at 1/500 dilution

    Lane 1 : NIH3T3 cell extract
    Lane 2 : NIH3T3 cell extract with immunising peptide


    Predicted band size : 115 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
  • ab59235 at 1/50 dilution staining Myosin Phosphatase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

参考文献

This product has been referenced in:
  • Wang XQ  et al. Resveratrol enhances vascular reactivity in mice following lipopolysaccharide challenge via the RhoA-ROCK-MLCP pathway. Exp Ther Med 14:308-316 (2017). Read more (PubMed: 28672931) »
  • Montalvo J  et al. ROCK1 & 2 perform overlapping and unique roles in angiogenesis and angiosarcoma tumor progression. Curr Mol Med 13:205-19 (2013). WB . Read more (PubMed: 22934846) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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