The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 115 kDa).
Regulates myosin phosphatase activity.
Expressed in striated muscles, specifically in type 2a fibers (at protein level).
Contains 6 ANK repeats.
Induced by 2-fold during pregnancy, including in abdominus rectus muscle.
Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
Cytoplasm. Along actomyosin filaments and stress fibers.
Fu K et al. Tra2ß Protein Is Required for Tissue-specific Splicing of a Smooth Muscle Myosin Phosphatase Targeting Subunit Alternative Exon. J Biol Chem287:16575-85 (2012).
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