製品の概要

  • 製品名Anti-Myosin light chain 3 antibody [MLM520]
    Myosin light chain 3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MLM520] to Myosin light chain 3
  • 特異性This antibody reacts with ventricle myosin light chain I.
  • アプリケーション適用あり: ELISA, IHC-Pmore details
  • 種交差性
    交差種: Rat, Cow, Human, Pig
  • 免疫原

    Human ventricle myosin light chain I.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Regulatory light chain of myosin. Does not bind calcium.
  • 関連疾患Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic type 8 (CMH8) [MIM:608751]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.
    Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 1 (MVC1) [MIM:608751]. MVC1 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
  • 配列類似性Contains 3 EF-hand domains.
  • 翻訳後修飾The N-terminus is blocked.
    N-terminus is methylated by METTL11A/NTM1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cardiac myosin light chain 1 antibody
    • CMH8 antibody
    • CMLC1 antibody
    • ELC of myosin antibody
    • Essential light chain of myosin antibody
    • MLC1SB antibody
    • MLC1V antibody
    • MYL3 antibody
    • MYL3_HUMAN antibody
    • Myosin light chain 1 antibody
    • Myosin light chain 1 slow twitch muscle B/ventricular isoform antibody
    • Myosin light chain 1 slow, B antibody
    • Myosin light chain 3 antibody
    • Myosin, light chain 1, ventricular antibody
    • myosin, light chain 3, alkali; ventricular, skeletal, slow antibody
    • myosin, light polypeptide 3, alkali antibody
    • myosin, light polypeptide 3, alkali; ventricular, skeletal, slow antibody
    • OTTHUMP00000165922 antibody
    • Slow skeletal ventricular myosin alkali light chain 3 antibody
    • slow-twitch muscle B/ventricular isoform antibody
    • Ventricular/slow twitch myosin alkali light chain antibody
    • VLC1 antibody
    see all

Anti-Myosin light chain 3 antibody [MLM520] 画像

  • Procedure:
    Coat microplate with porcine cardiac myosin light chain I.
    Wash 3 times
    Block with 1% BSA-PBS
    Add MLM520 diluted with BSA-PBS
    Incubate for 1 h at room temp.
    Wash 3 times
    Add HRP-labelled anti-mouse IgG diluted with 1% BSA-PBS
    Incubate for 1 h at room temp.
    Wash 3 times
    Add TMBZ solution.
    Incubate few min.
    Measure A450 nm.

  • ab679 (1µg/ml) staining myosin light chain in human heart (left ventricle), using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of cardiomyocytes.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-Myosin light chain 3 antibody [MLM520] (ab679) 使用論文

This product has been referenced in:
  • Berna M & Ackermann B Increased throughput for low-abundance protein biomarker verification by liquid chromatography/tandem mass spectrometry. Anal Chem 81:3950-6 (2009). ELISA . Read more (PubMed: 19388669) »
  • Berna MJ  et al. Strategic Use of Immunoprecipitation and LC/MS/MS for Trace-Level Protein Quantification: Myosin Light Chain 1, a Biomarker of Cardiac Necrosis. Anal Chem 79:4199-205 (2007). Read more (PubMed: 17447729) »

See all 2 Publications for this product

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