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Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Myosin Light Chain 2.
(Peptide available as ab88166.)
Our Abpromise guarantee covers the use of ab79935 in the following tested applications.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).|
|IHC-P||Use a concentration of 5 µg/ml.|
|IP||Use a concentration of 5 µg/ml.|
Myosin Light Chain 2 was immunoprecipitated using 0.5mg Mouse Heart tissue, 5µg of Rabbit polyclonal to Myosin Light Chain 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Heart tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79935.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 19kDa; Myosin Light Chain 2