Anti-Myosin Light Chain 2 抗体 (ab79935)

製品の概要

  • 製品名Anti-Myosin Light Chain 2 antibody
    Myosin Light Chain 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Myosin Light Chain 2
  • アプリケーション適用あり: ICC/IF, WB, IHC-P, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Sheep, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Myosin Light Chain 2.

    (Peptide available as ab88166.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissues: Human Heart; Human Skeletal Muscle; Mouse Heart; Rat Heart

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/140.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
IHC-P Use a concentration of 5 µg/ml.
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 関連疾患Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
  • 配列類似性Contains 3 EF-hand domains.
  • 翻訳後修飾N-terminus is methylated by METTL11A/NTM1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cardiac myosin light chain-2 antibody
    • Cardiac ventricular myosin light chain 2 antibody
    • CMH10 antibody
    • MLC 2v antibody
    • MLC-2 antibody
    • MLC-2v antibody
    • MLC2 antibody
    • MLRV_HUMAN antibody
    • MYL 2 antibody
    • MYL2 antibody
    • Myosin light chain 2 regulatory cardiac slow antibody
    • Myosin light polypeptide 2 regulatory cardiac slow antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2 ventricular/cardiac muscle isoform antibody
    • Regulatory light chain of myosin antibody
    • RLC of myosin antibody
    • Slow cardiac myosin regulatory light chain 2 antibody
    • ventricular/cardiac muscle isoform antibody
    see all

Anti-Myosin Light Chain 2 antibody 画像

  • Myosin Light Chain 2 was immunoprecipitated using 0.5mg Mouse Heart tissue, 5µg of Rabbit polyclonal to Myosin Light Chain 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Heart tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79935.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 19kDa; Myosin Light Chain 2

  • All lanes : Anti-Myosin Light Chain 2 antibody (ab79935) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 19 kDa
    Observed band size : 19 kDa
    Additional bands at : 17 kDa,8 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutes
  • IHC image of ab79935 staining in Mouse Heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79935, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ab79935 staining Myosin Light Chain 2 in Mouse embyronic (e18.5) cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% Serum for 1 hour 30 minutes at 20°C. Samples were incubated with primary antibody (1/140 in 1% goat serum + 10% BSA + 0.1% Triton X-100 in PBS) for 16 hours at 4°C. An AlexaFluor® 594-conjugated Goat anti-rabbit IgG (H+L) (1/200) was used as the secondary antibody.

    See Abreview

Anti-Myosin Light Chain 2 antibody (ab79935) 使用論文

ab79935 has not yet been referenced specifically in any publications.

Product Wall

Unfortunately, this is a customer review and both the data and the protocol information comes from the customer themselves. We therefore do not have any additional information regarding this image and what the green fluorescence corresponds to.
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryonic (E18.5) cardiomyocyte)
Specification Embryonic (E18.5) cardiomyocyte
Fixative Formaldehyde
Permeabilization Yes - 0.5% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 20°C
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投稿 Jun 18 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"