製品の概要

  • 製品名Anti-Myosin Light Chain 2 antibody
    Myosin Light Chain 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Myosin Light Chain 2
  • 特異性ab48003 is directed against the regulatory light chain of smooth and non muscle myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein.
  • アプリケーション適用あり: ELISA, WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mouse, Chicken, Cow, Dog, Human, Pig, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide:

    GANSNVFSMFEQTQIQ

    , corresponding to amino acids 12/27 of Human Myosin Light Chain 2

  • ポジティブ・コントロール
    • rat aorta

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab48003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000 - 1/20000.
WB 1/500 - 1/2000. Predicted molecular weight: 19 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 23704920
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 関連疾患Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
  • 配列類似性Contains 3 EF-hand domains.
  • 翻訳後修飾N-terminus is methylated by METTL11A/NTM1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cardiac myosin light chain-2 antibody
    • Cardiac ventricular myosin light chain 2 antibody
    • CMH10 antibody
    • MLC 2v antibody
    • MLC-2 antibody
    • MLC-2v antibody
    • MLC2 antibody
    • MLRV_HUMAN antibody
    • MYL 2 antibody
    • MYL2 antibody
    • Myosin light chain 2 regulatory cardiac slow antibody
    • Myosin light polypeptide 2 regulatory cardiac slow antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2 ventricular/cardiac muscle isoform antibody
    • Regulatory light chain of myosin antibody
    • RLC of myosin antibody
    • Slow cardiac myosin regulatory light chain 2 antibody
    • ventricular/cardiac muscle isoform antibody
    see all

Anti-Myosin Light Chain 2 antibody 画像

  • All lanes : Anti-Myosin Light Chain 2 antibody (ab48003) at 1/600 dilution

    Lane 1 : Vascular myosin (rat aorta), loaded with 35 µg of lysate
    Lane 2 : Cardiac myosin (mouse heart, loaded with 35 µg of lysate

    Secondary
    1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L]

    Predicted band size : 19 kDa
    Observed band size : 19,24 kDa (why is the actual band size different from the predicted?)
  • ab48003 (2µg/ml) staining Myosin Light Chain 2 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cell cytoplasm.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-Myosin Light Chain 2 antibody (ab48003) 使用論文

This product has been referenced in:
  • Christoforou N  et al. Transcription factors MYOCD, SRF, Mesp1 and SMARCD3 enhance the cardio-inducing effect of GATA4, TBX5, and MEF2C during direct cellular reprogramming. PLoS One 8:e63577 (2013). ICC/IF . Read more (PubMed: 23704920) »
  • Christoforou N  et al. Induced pluripotent stem cell-derived cardiac progenitors differentiate to cardiomyocytes and form biosynthetic tissues. PLoS One 8:e65963 (2013). Read more (PubMed: 23785459) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Cardiac cells)
Permeabilization Yes - 0.05% TritionX100
Specification Cardiac cells
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 16 2016

We recommend use of a 5% non fat dry milk/TBS blocking and incubation solution. We also recommend a starting dilution for the antibody within the range 1/500 - 1/2000. This is just a suggested starting range for the product. The optimal antibody diluti...

Read More

Thank you for your reply.

I can confirm that the immunogen for ab48003 was a synthetic peptide corresponding to amino acids 12 to 27 of Human Myosin Light Chain 2 (GANSNVFSMFEQTQIQ).

The antibody therefore binds to this region.
Read More

I have issued a free of charge replacement for one vial of ab92721. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abprom...

Read More

DISCOUNT CODE: *** Expiration date: April 6th, 2012 I am very pleased to hear you would like to accept our offer and test ab48003 in ICC on mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"