Anti-Myosin 抗体 [414] (ab8070)
Key features and details
- Mouse monoclonal [414] to Myosin
- Suitable for: IHC-Fr, ICC, IHC-P, WB
- Reacts with: Chicken
- Isotype: IgM
製品の概要
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製品名
Anti-Myosin antibody [414]
Myosin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [414] to Myosin -
由来種
Mouse -
特異性
This monoclonal antibody reacts in immunoblots specific with myosin and is specific to the 75kD of Myosin. Does not react with alpha-cardiac myosin. Does not react with the light-chain but with the last 75 kD of the myosin molecule. These 75 kD are located on a myosin-common site-chain. -
アプリケーション
適用あり: IHC-Fr, ICC, IHC-P, WBmore details -
種交差性
交差種: Chicken -
ポジティブ・コントロール
- Chicken muscle myomyofibrils.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.3
Preservative: 0.1% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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精製度
Tissue culture supernatant -
一次抗体 備考
This antibody, directed against myosin can be used for detection of myosin in frozen sections as well as in cultured cells. Applicable in paraffin sections only with microwave oven pretreatment. Also works in immunoblotting. -
ポリ/モノ
モノクローナル -
クローン名
414 -
アイソタイプ
IgM -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8070の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
Use at an assay dependent concentration.
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ICC |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
Use at 1/5 - 1/10.(Only with microwave oven pretreatment. Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher). Dilute immediately before use with PBS or Tris. |
|
WB |
Use at an assay dependent concentration.
|
特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Use at 1/5 - 1/10.(Only with microwave oven pretreatment. Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher). Dilute immediately before use with PBS or Tris. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Muscle contraction. -
配列類似性
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
ドメイン
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
細胞内局在
Cytoplasm > myofibril. Thick filaments of the myofibrils. - Information by UniProt
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別名
- adult 1 antibody
- MYH1 antibody
- MYH1_HUMAN antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab8070 は論文での使用が確認できていません。