製品の概要

  • 製品名Anti-Myosin antibody [414]
    Myosin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [414] to Myosin
  • 特異性This monoclonal antibody reacts in immunoblots specific with myosin and is specific to the 75kD of Myosin. Does not react with alpha-cardiac myosin. Does not react with the light-chain but with the last 75 kD of the myosin molecule. These 75 kD are located on a myosin-common site-chain.
  • アプリケーション適用あり: IHC-Fr, ICC, IHC-P, WBmore details
  • 種交差性
    交差種: Chicken
  • ポジティブ・コントロール
    • Chicken muscle myomyofibrils.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 0.1% Sodium azide
    Constituent: 1% BSA
  • 精製度Tissue culture supernatant
  • 一次抗体 備考This antibody, directed against myosin can be used for detection of myosin in frozen sections as well as in cultured cells. Applicable in paraffin sections only with microwave oven pretreatment. Also works in immunoblotting.
  • ポリ/モノモノクローナル
  • クローン名414
  • アイソタイプIgM
  • 軽鎖の種類unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Use at 1/5 - 1/10.(Only with microwave oven pretreatment. Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher). Dilute immediately before use with PBS or Tris.

WB Use at an assay dependent concentration.

ターゲット情報

  • 機能Muscle contraction.
  • 配列類似性Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • ドメインThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • 細胞内局在Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt
  • 別名
    • adult 1 antibody
    • MYH1 antibody
    • MYH1_HUMAN antibody
    • MYH2 antibody
    • MYH3 antibody
    • MYH4 antibody
    • MYH6 antibody
    • MyHC-2x antibody
    • MyHC-IIx/d antibody
    • MYL1 antibody
    • MYL2 antibody
    • MYL3 antibody
    • MYL5 antibody
    • MYL6 antibody
    • Myosin light chain 2 regulatory cardiac slow antibody
    • Myosin 1 antibody
    • Myosin heavy chain 1 antibody
    • Myosin heavy chain 1 skeletal muscle adult antibody
    • Myosin heavy chain 2 skeletal muscle adult antibody
    • Myosin heavy chain 2x antibody
    • Myosin heavy chain 3 skeletal muscle adult antibody
    • Myosin heavy chain 4 skeletal muscle adult antibody
    • Myosin heavy chain 6 skeletal muscle adult antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain IIx/d antibody
    • Myosin heavy chain skeletal muscle adult 1 antibody
    • Myosin II antibody
    • Myosin light chain 1 alkali skeletal fast antibody
    • Myosin light chain 3 alkali ventricular skeletal slow antibody
    • Myosin light chain 4 alkali atrial embryonic antibody
    • Myosin light chain 5 regulatory antibody
    • Myosin light chain 6 alkali smooth muscle and non muscle antibody
    • Myosin-1 antibody
    • Myosin1 antibody
    • skeletal muscle antibody
    see all

Anti-Myosin antibody [414] (ab8070) 使用論文

ab8070 has not yet been referenced specifically in any publications.

Product Wall

Thank you for responding so promptly. I'm sorry to hear you're not happy with my offer. At Abcam we value each and every one of our customers. However, I must stand by our policy. We do not routinely offer free or trial sized samples for testing purpo...

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Thank you for your inquiry. Yes, the antibody can be used for smoothe muscle.

Thanks you for your message. The deadline for placing orders to be dispatched before the holidays is midday December 18th. Please do let me know how soon you need the products. Would it be all right at the beginning of January? Or is it too late? ...

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This antibody will react with muscle and heart tissue, however it will not react with alpha-cardiac myosin. Furthermore this antibody has only been tested on human tissue and we suspect that it will not cross-react with mouse and/or rat although this h...

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All the information we have for antibodies to myosin, (ab7784, ab7829, ab7786, ab7785,) is in the datasheets

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"