製品の概要

  • 製品名Anti-Myosin 1G antibody
  • 製品の詳細
    Rabbit polyclonal to Myosin 1G
  • アプリケーション適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen, corresponding to amino acids 271-356 (SVHRILAAIL HLGNIEFVET EEGGLQKEGL AVAEEALVDH VAELTATPRD LVLRSLLART VASGGRELIE KGHTAAEASY ARDACA) of Human Myosin 1G.

  • ポジティブ・コントロール
    • Human lymph node tissue

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments.
    Precursor of the minor histocompatibility antigen HA-2. More generally, minor histocompatibility antigens (mHags) refer to immunogenic peptide which, when complexed with MHC, can generate an immune response after recognition by specific T-cells. The peptides are derived from polymorphic intracellular proteins, which are cleaved by normal pathways of antigen processing. The binding of these peptides to MHC class I or class II molecules and their expression on the cell surface can stimulate T-cell responses and thereby trigger graft rejection or graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation from HLA-identical sibling donor. GVHD is a frequent complication after bone marrow transplantation (BMT), due to mismatch of minor histocompatibility antigen in HLA-matched sibling marrow transplants. HA-2 is restricted to MHC class I HLA-A*0201.
  • 組織特異性Specifically expressed in hematopoietic cells.
  • 配列類似性Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • 細胞内局在Cell membrane. Localization at the membrane is not highly dependent on phosphatidylinositol 4,5-bisphosphate levels. Released from the membrane in the presence of ATP. May be enriched in peripheral processes, such as microvilli or ruffles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • mHag HA-2 antibody
    • Minor histocompatibility antigen HA-2 antibody
    • Myo1g antibody
    • MYO1G_HUMAN antibody
    • myosin IG antibody
    see all

Anti-Myosin 1G antibody 画像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab121657 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121657, at 1/150 dilution, staining Myosin 1G in paraffin-embedded Human lymph node tissue by Immunohistochemistry.

Anti-Myosin 1G antibody (ab121657) 使用論文

ab121657 has not yet been referenced specifically in any publications.

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