製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Myosin 18B
  • アプリケーション
    適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 2420-2564 of Human (Q8IUG5).

  • ポジティブ・コントロール
    • Human kidney tissue, A431 cells

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

ターゲット情報

  • 機能
    May be involved in intracellular trafficking of the muscle cell when in the cytoplasm, whereas entering the nucleus, may be involved in the regulation of muscle specific genes. May play a role in the control of tumor development and progression; restored MYO18B expression in lung cancer cells suppresses anchorage-independent growth.
  • 組織特異性
    Selectively expressed in cardiac and skeletal muscles. Weakly expressed in testis, pancreas, placenta, prostate, lung and thymus.
  • 配列類似性
    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • 発生段階
    Reaches an expression peak in the third day after induction and remains at similar level during successive myotubule maturation.
  • 細胞内局在
    Cytoplasm. Nucleus. Cytoplasm > myofibril > sarcomere. Punctate pattern in undifferentiated myoblasts. Nuclear, on primary cardiomyocytes and adult muscle. A partial sarcomeric location was found in some cardiomyocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BK125H2.1 antibody
    • DKFZp434C2422 antibody
    • DKFZp779C1668 antibody
    • MY18B_HUMAN antibody
    • MYO18B antibody
    • myosin 18B antibody
    • myosin XVIIIB antibody
    • Myosin-XVIIIb antibody
    see all

画像

  • ab121125 at 1/10 dilution staining Myosin 18B in Paraffin-emebedded Human kidney tissue by Immunohistochemistry. Note: moderate cytoplasmic positivity in renal tubules.
  • Immunofluorescent staining of human cell line A431 using ab121125 at a concentration of 4 µg/ml. Note: shows positivity in nucleus but not nucleoli & cytoplasm.

参考文献

ab121125 has not yet been referenced specifically in any publications.

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