製品の概要

  • 製品名Anti-Myoglobin antibody
    Myoglobin 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to Myoglobin
  • アプリケーション適用あり: WBmore details
  • 種交差性

    交差が予測される動物種: Dog, Human, Pig, Chimpanzee
  • 免疫原

    Recombinant fusion protein: MGLSDGEWHL VLNVWGKVET DLAGHGQEVL IRLFKSHPET LEKFDKFKHL KSEDDMRRSE DLRKHGNTVL TALGGILKKK GHHEAELKPL AQSHATKHKI , corresponding to amino acids 1-100 of Grey seal (Halichoerus grypus) Myoglobin

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab43127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 17 kDa.

ターゲット情報

Anti-Myoglobin antibody 画像

  • All lanes : Anti-Myoglobin antibody (ab43127) at 1/1000 dilution

    Lane 1 : Total protein extract from E.coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen
    Lane 2 : Total protein extract from E.coli with ~50ng to 500ng of the antigen fusion protein

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa

Anti-Myoglobin antibody (ab43127) 使用論文

This product has been referenced in:
  • Vázquez-Medina JP  et al. Apnea stimulates the adaptive response to oxidative stress in elephant seal pups. J Exp Biol 214:4193-200 (2011). WB . Read more (PubMed: 22116762) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"