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Ni-NTA purified truncated recombinant Myoglobin expressed in E. Coli strain BL21 (DE3)
This product was changed from ascites to supernatant. Lot no’s high than GR232969-1 are from Tissue Culture Supernatant
Our Abpromise guarantee covers the use of ab47702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 17 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||1/500 - 1/2000.|
ab47702 staining Myoglobin in cow skeletal muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed by heat fixation and blocked with 5% serum for 30 minutes at 23°C. Samples were incubated with primary antibody (1/2000) for 1 hour at 23°C. An Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Green - dystrophin, Blue - Hoechst.