製品の概要

  • 製品名
    Anti-Myogenin antibody [F5D]
    Myogenin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [F5D] to Myogenin
  • アプリケーション
    適用あり: Flow Cyt, ICC/IF, IHC-P, WB, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
  • 免疫原

    BALB/ C mice were injected with recombinant protein containing rat myogenin amino acid 30 - 224.

  • ポジティブ・コントロール
    • Rhabdomyosarcoma

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50.
ICC/IF 1/50.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Recommended starting point for ab1835 incubation is 30min RT.
WB 1/250. Detects a band of approximately 40 kDa (predicted molecular weight: 25 kDa).

Abcam recommends using BSA as the blocking agent.

IHC-FoFr Use a concentration of 1 µg/ml.

ターゲット情報

画像

  • All lanes : Anti-Myogenin antibody [F5D] (ab1835) at 1/250 dilution

    Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 2 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab1835 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

  • Adult mouse muscle section stained with  ab1835. The animals were perfused with 4% PFA. The sections were incubated in 5% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab1835, 1µg/ml) overnight at +4°C. The secondary antibody Alexa Fluor® 568 donkey anti-mouse IgG (H+L) (red) was used at a 1/1000 dilution for 1h.
  • ab1835 at 1/50 dilution staining pig differentiated skeletal myoblast cells by ICC/IF. The cells were paraformaldehyde fixed, permeabilized with Triton-X100 and incubated with the antibody overnight at 4C. An Alexa Fluor 488 conjugated goat anti-mouse antibody was used as the secondary. The image was captured using a confocal laser scanning microscope with an additional Differential Interference Contrast (DIC) mode. The image shows DAPI nuclear counterstain (blue, upper left panel), Myogenin staining (green, upper right panel), DIC (phase) image (lower left panel) and overlay (lower right panel).

    Myogenin is not expressed in proliferating myoblasts, but rather in mononucleated differentiating myoblasts (See abreview for more detail).

    See Abreview

  • ab1835 - immunohistochemistry

    Formalin fixed paraffin embedded human rhabdomyosarcoma stained with Myogenin using ABC and AEC chromogen.

参考文献

This product has been referenced in:
  • Xu X  et al. LncRNA H19 promotes the differentiation of bovine skeletal muscle satellite cells by suppressing Sirt1/FoxO1. Cell Mol Biol Lett 22:10 (2017). WB ; Cow . Read more (PubMed: 28652859) »
  • Furukawa S  et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Rat . Read more (PubMed: 28190929) »

See all 29 Publications for this product

レビューと Q&A

Application
Flow Cytometry
Sample
Pig Cell (Muscle)
Permeabilization
Yes - 0.1% Triton X-100 / PBS
Gating Strategy
Negative control (cells incubated with normal serum instead of primary antibody)
Specification
Muscle
Preparation
Cell harvesting/tissue preparation method: Cells detached with HyQtase, 10% FBS / DPBS added, centrifugation of cells, fixation in 4% PFA (30 min, 4°C) wash with PBS 3 times, permeabilization 0.1% Triton X-100 / PBS (10 min, RT) blocking 2% rabbit serum / 0.1% Triton X-100 / PBS (60 min, RT) incubation with antibody
Sample buffer: 2% Rabbit Serum / 0.1% Triton X-100 / PBS
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 26 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (skeletal muscle)
Loading amount
30 µg
Specification
skeletal muscle
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 Jan 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Muscles)
Specification
Muscles
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX100
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 May 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Muscle)
Loading amount
20 µg
Specification
Muscle
Gel Running Conditions
Reduced Denaturing (12% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Mar 24 2010

Application
Western blot
Sample
Human Cell lysate - whole cell (skeletal myotubes)
Loading amount
5 µg
Specification
skeletal myotubes
Gel Running Conditions
Reduced Denaturing (10 % gel, semi-dry)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Henrike Sell

Verified customer

投稿 Feb 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Differentiated skeletal myoblast)
Specification
Differentiated skeletal myoblast
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Dr. Mal Niladri

Verified customer

投稿 Oct 16 2006

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (skeletal myoblast transplanted heart tissue)
Specification
skeletal myoblast transplanted heart tissue
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Username

Dr. Mal Niladri

Verified customer

投稿 Oct 09 2006

This antibody has only been shown to positively stain the nuclei of myoblasts from human fetal tissue but no reactivity has been observed in adult skeletal muscle. We generally use this antibody in-house for detecting myogenin in the nuclei of rhabdom...

Read More

Myogenin is expressed in both normal and tumor cells. Myogenin is expressed in high levels in fetal skeletal muscle, and this antibody has been used to stain human, mouse, and rat cultured myotubes and neonatal mouse, rat, and cat tissue. This clone ...

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Thank you for your enquiry. Ab1835 has been tested for application in Western blotting and shown to cross-react with rat, mouse, and cat (it has not yet been tested in other species). For this antibody in Western blotting, we don't have a specific dilu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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