製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Myogenin
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    3 synthetic peptides (human) conjugated to KLH

  • ポジティブ・コントロール
    • Induced culture of E.coli (one shot Top10 competent cells).

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    ELISA: Use at an assay dependent dilution.
    Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/40000 is determined for one of the three peptides.
    WB: 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
    • 配列類似性
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • bHLHc3 antibody
      • cb553 antibody
      • Class C basic helix-loop-helix protein 3 antibody
      • MYF 4 antibody
      • Myf-4 antibody
      • MYF4 antibody
      • MYOG antibody
      • MYOG_HUMAN antibody
      • Myogenic factor 4 antibody
      • Myogenic factor 4 myogenin antibody
      • Myogenin antibody
      • Myogenin myogenin factor 4 antibody
      • OTTHUMP00000039094 antibody
      see all

    Anti-Myogenin antibody 画像

    • All lanes : Anti-Myogenin antibody (ab82843) at 1/1000 dilution

      Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
      Lane 2 : induced culture of E.coli (one shot Top10 competent cells)

      Secondary
      anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 39 kDa
      Observed band size : 39 kDa

    プロトコール

    Anti-Myogenin antibody (ab82843) 使用論文

    ab82843 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (Muscle (gastrocnemius))
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    35 µg
    Specification
    Muscle (gastrocnemius)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Ms. Sandra Pérez

    Verified customer

    投稿 Apr 20 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Skeletal Muscle)
    Loading amount
    30 µg
    Specification
    Skeletal Muscle
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 12 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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