Anti-Myogenin 抗体 (ab103924)
Key features and details
- Goat polyclonal to Myogenin
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Myogenin antibody
Myogenin 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Myogenin -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Sheep, Rabbit, Goat, Cow, Dog, Human, Pig -
免疫原
Synthetic peptide:
C-TDAHNLHSLTSIVD
, corresponding to C terminal amino acids 193-206 of Human Myogenin (NP_002470.2). -
ポジティブ・コントロール
- Recombinant Human Myogenin protein (ab114244) can be used as a positive control in WB. Mouse Skeletal Muscle lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab103924 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab103924の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 25 kDa).
A 1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 25 kDa). A 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 281343 Cow
- Entrez Gene: 490224 Dog
- Entrez Gene: 4656 Human
- Entrez Gene: 17928 Mouse
- Entrez Gene: 497618 Pig
- Entrez Gene: 29148 Rat
- Entrez Gene: 443158 Sheep
- Omim: 159980 Human
see all -
別名
- bHLHc3 antibody
- cb553 antibody
- Class C basic helix-loop-helix protein 3 antibody
see all
画像
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Anti-Myogenin antibody (ab103924) at 2 µg/ml + Mouse Skeletal Muscle lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: ~38 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (2)
ab103924 は 2 報の論文で使用されています。
- Rodriguez BL et al. A Comparison of Ovine Facial and Limb Muscle as a Primary Cell Source for Engineered Skeletal Muscle. Tissue Eng Part A 26:167-177 (2020). PubMed: 31469044
- Zhang W et al. Transcription factor EGR1 promotes differentiation of bovine skeletal muscle satellite cells by regulating MyoG gene expression. J Cell Physiol 233:350-362 (2018). PubMed: 28256014