Anti-Myoferlin 抗体 [7D2] (ab76746)
Key features and details
- Mouse monoclonal [7D2] to Myoferlin
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-Myoferlin antibody [7D2]
Myoferlin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7D2] to Myoferlin -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide corresponding to Human Myoferlin (N terminal).
Database link: Q9NZM1 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
7D2 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76746の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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Flow Cyt | (1) |
1/100.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
Use a concentration of 5 µg/ml. |
Flow Cyt
1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR. -
組織特異性
Expressed in myoblast and endothelial cells (at protein level). Highly expressed in cardiac and skeletal muscles. Also present in lung, and at very low levels in kidney, placenta and brain. -
配列類似性
Belongs to the ferlin family.
Contains 5 C2 domains. -
ドメイン
The C2 domain 1 associates with lipid membranes in a calcium-dependent manner. -
細胞内局在
Cell membrane. Nucleus membrane. Cytoplasmic vesicle membrane. Concentrated at the membrane sites of both myoblast-myoblast and myoblast-myotube fusions. Detected at the plasmalemma in endothelial cells lining intact blood vessels (By similarity). Found at nuclear and plasma membranes. Enriched in undifferentiated myoblasts near the plasma membrane in puncate structures. - Information by UniProt
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参照データベース
- Entrez Gene: 26509 Human
- Entrez Gene: 226101 Mouse
- Omim: 604603 Human
- SwissProt: Q9NZM1 Human
- SwissProt: Q69ZN7 Mouse
- Unigene: 602086 Human
- Unigene: 34674 Mouse
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別名
- Fer 1 like 3, myoferlin (C. elegans) antibody
- Fer 1 like family member 3 antibody
- Fer 1 like protein 3 antibody
see all
画像
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ICC/IF image of ab76746 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76746, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Overlay histogram showing HeLa cells stained with ab76746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76746, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab76746 は 8 報の論文で使用されています。
- Na JM et al. Correlation between myoferlin expression and lymph node metastasis in papillary thyroid carcinoma. J Pathol Transl Med 56:199-204 (2022). PubMed: 35535365
- Kiselev A et al. Truncating Variant in Myof Gene Is Associated With Limb-Girdle Type Muscular Dystrophy and Cardiomyopathy. Front Genet 10:608 (2019). PubMed: 31297131
- An HJ et al. Myoferlin silencing inhibits VEGFR2-mediated proliferation of metastatic clear cell renal cell carcinoma. Sci Rep 9:12656 (2019). PubMed: 31477752
- Song DH et al. Prognostic role of myoferlin expression in patients with clear cell renal cell carcinoma. Oncotarget 8:89033-89039 (2017). IHC-P ; Human . PubMed: 29179496
- Piper AK et al. Enzymatic cleavage of myoferlin releases a dual C2-domain module linked to ERK signalling. Cell Signal 33:30-40 (2017). PubMed: 28192161
- Song DH et al. Myoferlin expression in non-small cell lung cancer: Prognostic role and correlation with VEGFR-2 expression. Oncol Lett 11:998-1006 (2016). IHC . PubMed: 26893682
- Lund TC et al. sdf1 Expression reveals a source of perivascular-derived mesenchymal stem cells in zebrafish. Stem Cells 32:2767-79 (2014). Flow Cyt ; Zebrafish . PubMed: 24905975
- Leung C et al. Expression of myoferlin in human airway epithelium and its role in cell adhesion and zonula occludens-1 expression. PLoS One 7:e40478 (2012). IHC-P, WB, ICC/IF ; Human . PubMed: 22808170