製品の概要

  • 製品名
    Anti-MyoD1 antibody [5.2F]
    MyoD1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5.2F] to MyoD1
  • 特異性
    This antibody does not cross react with Myogenin, Myf5, or Myf6.
  • アプリケーション
    適用あり: ICC/IF, Sandwich ELISA, ELISA, IP, IHC-P, IHC-Fr, Electron Microscopy, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human
    交差が予測される動物種: Sheep, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to Mouse MyoD1 aa 3-56 (N terminal).
    Sequence:

    LLSPPLRDIDLTGPDGSLCSFETADDFYDDPCFDSPDLRFFEDLDPRLVH VGAL

  • ポジティブ・コントロール
    • Rhabdomyosarcoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MyoD1 (ab64159). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to MyoD1 (ab64159) as Detection.
ELISA Use at an assay dependent concentration.
IP Use at 2 µg/mg of lysate.
IHC-P Use a concentration of 2 - 4 µg/ml.
IHC-Fr Use a concentration of 2 - 4 µg/ml.
Electron Microscopy Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.

ターゲット情報

Anti-MyoD1 antibody [5.2F] 画像

  • All lanes : Anti-MyoD1 antibody [5.2F] (ab16148) at 1/1000 dilution

    Lane 1 : Mouse muscle tissue lysate
    Lane 2 : Mouse liver tissue lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated goat anti-mouse IgG polyclonal at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 35 kDa


    Exposure time : 20 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab16148 staining MyoD1 in rat differentiated skeletal muscle cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde and then blocked using 2% serum for 1 hour. Samples were then incubated with primary antibody at 1/200 for 8 hours. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue).
  • Immunohistochemical staining using ab16148  at 2ug/ml on formalin fixed, paraffin embedded samples of human rhabdomyosarcoma.

  • Standard Curve for Myo-D; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [5.2F] to MyoD1 (ab16148) at 5ug/ml and Detector Antibody Rabbit polyclonal to MyoD1 (ab64159) at 0.1ug/ml.

Anti-MyoD1 antibody [5.2F] (ab16148) 使用論文

This product has been referenced in:
  • Tenente IM  et al. Myogenic regulatory transcription factors regulate growth in rhabdomyosarcoma. Elife 6:N/A (2017). Read more (PubMed: 28080960) »
  • Tanaka N  et al. Acquired platinum resistance involves epithelial to mesenchymal transition through ubiquitin ligase FBXO32 dysregulation. JCI Insight 1:e83654 (2016). Read more (PubMed: 27812537) »

See all 14 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Tissue lysate - whole (Muscle (gastrocnemius))
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
35 µg
Specification
Muscle (gastrocnemius)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 20 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
Muscle
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 08 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Sheep Cell (myoblast/other muscle precursor)
Permeabilization
No
Specification
myoblast/other muscle precursor
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 4°C
Fixative
Methanol
Username

Abcam user community

Verified customer

投稿 Aug 31 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (Muscle of leg)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Cytrate
Permeabilization
No
Specification
Muscle of leg
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Username

Antoine Veron

Verified customer

投稿 Jul 12 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Myocyte of blood vessel)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Cytrate
Permeabilization
No
Specification
Myocyte of blood vessel
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Username

Antoine Veron

Verified customer

投稿 Jul 12 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Pig Cell (muscle, satellite cell)
Permeabilization
No
Gating Strategy
negative control (cells incubated with normal serum instead of primary antibody)
Specification
muscle, satellite cell
Preparation
Cell harvesting/tissue preparation method: cells detached with HyQtase, 10%FBS/DPBS added, after centrifugation pellet resuspended in 1mM EDTA/PBS, fixed in methanol, washed with sample buffer
Sample buffer: 5 mM EDTA, 1% BSA in DPBS
Fixation
Methanol
Username

Abcam user community

Verified customer

投稿 Jan 28 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (muscle, satellite cell)
Permeabilization
No
Specification
muscle, satellite cell
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 28 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Fish Tissue sections (Skeletal muscle fish of Pseudoplatystoma fasciatum)
Antigen retrieval step
None
Permeabilization
No
Specification
Skeletal muscle fish of Pseudoplatystoma fasciatum
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Acetone
Username

Dr. Danilo Aguiar

Verified customer

投稿 May 27 2015

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 31 2014

I believe that you are referring to the data in rat cells sent to us from one of our customers(http://www.abcam.com/MyoD1-antibody-52F-ab16148/reviews/6194)

As Abcam has not produced this image ourselves we do not have any additional informat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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