製品の概要

  • 製品名Anti-Myocardin antibody
    Myocardin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Myocardin
  • アプリケーション適用あり: WB, IHC-Frmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal domain of Human Myocardin.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab106346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200 - 1/2000. Predicted molecular weight: 102 kDa.
IHC-Fr 1/50 - 1/500.

ターゲット情報

  • 機能Transcriptional factor that uses the canonical single or multiple CArG boxes DNA sequence. Binds CArG boxes only in the presence of serum response factor (SRF). Acts as a cofactor of SRF and modulates SRF-target genes. Regulates the expression of a set of cardiac and smooth muscle-specific genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage.
  • 組織特異性Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.
  • 配列類似性Contains 3 RPEL repeats.
    Contains 1 SAP domain.
  • ドメインThe C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MYCD antibody
    • MYCD_HUMAN antibody
    • Myocardin antibody
    • Myocd antibody

Anti-Myocardin antibody (ab106346) 使用論文

ab106346 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (pulmonary artery smooth muscle cell)
Loading amount 14 µg
Specification pulmonary artery smooth muscle cell
Treatment both treated and not treated by anti-mir145 30nM for 4 days
Gel Running Conditions Non-reduced Denaturing (10% BisTris Gel)
Blocking step Licor Blocking buffer as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 4°C
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投稿 Sep 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"