Anti-MYLIP 抗体 (ab107168)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MYLIP
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Macaque monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human MYLIP.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293; HepG2; SHSY5Y; NIH3T3.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab107168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).

ターゲット情報

  • 機能
    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
  • 組織特異性
    Ubiquitously expressed.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Contains 1 FERM domain.
    Contains 1 RING-type zinc finger.
  • 発生段階
    Expressed in fetal tissues and higher levels were detected in placenta and fetal lung.
  • ドメイン
    The RING domain mediates ubiquitination and the neurite outgrowth inhibitory activity.
  • 翻訳後修飾
    Autoubiquitinated.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BM-023 antibody
    • BZF1 antibody
    • E3 ubiquitin protein ligase MYLIP antibody
    • E3 ubiquitin-protein ligase MYLIP antibody
    • Idol antibody
    • Inducible degrader of the LDL-receptor antibody
    • Lipoprotein receptor antibody
    • MIR antibody
    • MYLIP antibody
    • MYLIP_HUMAN antibody
    • Myosin regulatory light chain interacting protein antibody
    • PP5242 antibody
    see all

Anti-MYLIP antibody 画像

  • All lanes : Anti-MYLIP antibody (ab107168) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

プロトコール

Anti-MYLIP antibody (ab107168) 使用論文

ab107168 has not yet been referenced specifically in any publications.

Product Wall

Thank you for submitting an Abreview of ab107168. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録