This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293; HepG2; SHSY5Y; NIH3T3.
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1/250. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
Protein modification; protein ubiquitination.
Contains 1 FERM domain. Contains 1 RING-type zinc finger.
Expressed in fetal tissues and higher levels were detected in placenta and fetal lung.
The RING domain mediates ubiquitination and the neurite outgrowth inhibitory activity.