製品の概要

  • 製品名
    Anti-Mylc2b (phospho S18) antibody
  • 製品の詳細
    Rabbit polyclonal to Mylc2b (phospho S18)
  • 特異性
    ab63479 detects Mylc2b only when phosphorylated at serine 18 (Human: Ser18; Mouse: Ser20; Rat: Ser19).
  • アプリケーション
    適用あり: IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide (Human) derived from Mylc2b from around the phosphorylation site of serine 18 (A-T-SP-N-V).

  • ポジティブ・コントロール
    • Human brain tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab63479 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab63479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
ELISA 1/20000.

ターゲット情報

  • 関連性
    Mylc2b (Myosin regulatory light chain 2 B, smooth muscle isoform) plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity.
  • 参照データベース
  • 別名
    • Myosin light chain regulatory B antibody
    • Myosin regulatory light chain 2 B, smooth muscle isoform antibody
    • RLC B antibody

画像

  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using Mylc2b (phospho S18) antibody (ab63479) at 1/50 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.

プロトコール

参考文献

This product has been referenced in:
  • Wu F  et al. Identification of phosphorylated MYL12B as a potential plasma biomarker for septic acute kidney injury using a quantitative proteomic approach. Int J Clin Exp Pathol 8:14409-16 (2015). Read more (PubMed: 26823757) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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