製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MYL9
  • アプリケーション
    適用あり: WB, IP, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Bird, Mammal
  • 免疫原

    Synthetic peptide:

    KKRPQRATSNVFAMFD

    , corresponding to amino acids 12-27 of Human MYL9

  • ポジティブ・コントロール
    • Colon, Smooth Muscle tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab64161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 20 kDa.
IP Use at an assay dependent concentration.
ELISA 1/5000 - 1/20000.
IHC-P Use a concentration of 2.5 µg/ml.

ターゲット情報

  • 機能
    Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
  • 組織特異性
    Smooth muscle tissues and in some, but not all, nonmuscle cells.
  • 配列類似性
    Contains 3 EF-hand domains.
  • 翻訳後修飾
    Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 20 kDa myosin light chain antibody
    • Human 20kDa myosin light chain (MLC2) mRNA complete cds antibody
    • LC20 antibody
    • MGC3505 antibody
    • MLC 2 antibody
    • MLC-2C antibody
    • MLC2 antibody
    • MLY 9 antibody
    • MRLC1 antibody
    • MYL9 antibody
    • MYL9_HUMAN antibody
    • Myosin light chain 9 regulatory antibody
    • Myosin light polypeptide 9 regulatory antibody
    • myosin regulatory light chain 1 antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2 smooth muscle isoform antibody
    • Myosin regulatory light chain 9 antibody
    • Myosin regulatory light chain MRLC1 antibody
    • Myosin regulatory light polypeptide 9 antibody
    • Myosin RLC antibody
    • Myosin vascular smooth muscle light chain 2 antibody
    • MYRL2 antibody
    • OTTHUMP00000030857 antibody
    • smooth muscle isoform antibody
    see all

画像

  • ab64161 at 2.5µg/ml staining MYL9 in Colon, Smooth Muscle by Immunohistochemistry, Formalin-fixed, paraffin-embedded tissue.
  • Anti-MYL9 antibody (ab64161) at 1/2000 dilution + Recombinant Human MYL9 protein (ab104014) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

参考文献

This product has been referenced in:
  • Hudson CA  et al. Phasic contractions of isolated human myometrium are associated with Rho-kinase (ROCK)-dependent phosphorylation of myosin phosphatase-targeting subunit (MYPT1). Mol Hum Reprod 18:265-79 (2012). WB . Read more (PubMed: 22155728) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Myometrial smooth muscle cells)
Loading amount
10 µg
Specification
Myometrial smooth muscle cells
Gel Running Conditions
Reduced Denaturing (10% Bis-Tris gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Jun 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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