製品の概要

  • 製品名
    Anti-MYH4 antibody
  • 製品の詳細
    Rabbit polyclonal to MYH4
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from Human MYH4 at C-terminal

  • ポジティブ・コントロール
    • Human skeletal muscle tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab111442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Muscle contraction.
  • 配列類似性
    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • ドメイン
    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • 細胞内局在
    Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt
  • 参照データベース
  • 別名
    • fetal antibody
    • MYH2B antibody
    • Myh4 antibody
    • MYH4_HUMAN antibody
    • MyHC-2b antibody
    • MyHC-IIb antibody
    • Myosin heavy chain 2b antibody
    • Myosin heavy chain 4 antibody
    • Myosin heavy chain 4 skeletal muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain IIb antibody
    • Myosin heavy chain skeletal muscle fetal antibody
    • Myosin-4 antibody
    • skeletal muscle antibody
    see all

画像

  • ab111442, at 1/50 dilution, staining MYH4 in paraffin-embedded Human skeletal muscle tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

プロトコール

参考文献

ab111442 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Pig Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 500µg/mL · Temperature: 34°C
Username

Md Shamimul Hasan

Verified customer

投稿 Jul 20 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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