Anti-Myf5 抗体 [EPR4899] (ab125078)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4899] to Myf5
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Myf5 antibody [EPR4899]
Myf5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4899] to Myf5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Pig -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human skeletal muscle, rat muscle, mouse muscle and rat heart tissue lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4899 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125078の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 37 kDa (predicted molecular weight: 28 kDa).
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IP |
1/20.
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特記事項 |
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WB
1/10000 - 1/50000. Detects a band of approximately 37 kDa (predicted molecular weight: 28 kDa). |
IP
1/20. |
ターゲット情報
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機能
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4617 Human
- Entrez Gene: 17877 Mouse
- Entrez Gene: 407603 Pig
- Entrez Gene: 299766 Rat
- Omim: 159990 Human
- SwissProt: P13349 Human
- SwissProt: P24699 Mouse
- Unigene: 178023 Human
see all -
別名
- bHLHc2 antibody
- Class C basic helix loop helix protein 2 antibody
- Class C basic helix-loop-helix protein 2 antibody
see all
画像
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Anti-Myf5 antibody [EPR4899] (ab125078) at 1/50000 dilution (purified) + Human skeletal muscle tissue lysate at 10 µg
Secondary
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST -
All lanes : Anti-Myf5 antibody [EPR4899] (ab125078) at 1/50000 dilution (purified)
Lane 1 : Rat muscle tissue lysate
Lane 2 : Mouse muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST -
ab125078 (Purified) at 1/20 dilution (20 µg/ml) immunoprecipitating Myf5 in Human skeletal muscle lysate.
Lane 1 (input): Human skeletal muscle
Lane 2 (+): 125078 & Human skeletal muscle lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125078 in human skeletal muscle lysate
For western blotting, ab125078 at 1/1000 and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST. -
All lanes : Anti-Myf5 antibody [EPR4899] (ab125078) at 1/10000 dilution (unpurified)
Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : Rat muscle tissue lysate
Lane 3 : Mouse muscle tissue lysate
Lane 4 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 28 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab125078 は 11 報の論文で使用されています。
- Yang C et al. Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation. Mol Ther Nucleic Acids 23:872-886 (2021). PubMed: 33614236
- Zheng Y et al. Integrated analysis of long non-coding RNAs (lncRNAs) and mRNA expression profiles identifies lncRNA PRKG1-AS1 playing important roles in skeletal muscle aging. Aging (Albany NY) 13:15044-15060 (2021). PubMed: 34051073
- Chaudhary P et al. High altitude mediated skeletal muscle atrophy: Protective role of curcumin. Biochimie 156:138-147 (2019). PubMed: 30347230
- Stewart E et al. Identification of Therapeutic Targets in Rhabdomyosarcoma through Integrated Genomic, Epigenomic, and Proteomic Analyses. Cancer Cell 34:411-426.e19 (2018). PubMed: 30146332
- Boularaoui SM et al. Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle. J Tissue Eng Regen Med 12:e918-e936 (2018). PubMed: 28101909