製品の概要

  • 製品名Anti-Myf5 antibody
    Myf5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Myf5
  • 特異性Detects endogenous levels of total Myf5 protein. Cross-reactivity with Myf5, MyoD and Myf6 (greater than 85% sequence homology).
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthesized peptide derived from an internal sequence of human Myf5.

  • ポジティブ・コントロール
    • Extracts from HepG2 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
ELISA 1/40000.

ターゲット情報

Anti-Myf5 antibody 画像

  • All lanes : Anti-Myf5 antibody (ab69997) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells, minus immunising peptide
    Lane 2 : Extracts from HepG2 cells, plus immunising peptide at 5µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 28 kDa
    Observed band size : 28 kDa

Anti-Myf5 antibody (ab69997) 使用論文

This product has been referenced in:
  • Bareja A  et al. Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis. PLoS One 9:e90398 (2014). ICC ; Mouse, Human . Read more (PubMed: 24587351) »
  • Koch AJ & Holaska JM Loss of Emerin Alters Myogenic Signaling and miRNA Expression in Mouse Myogenic Progenitors. PLoS One 7:e37262 (2012). WB . Read more (PubMed: 22606356) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Rabbit Tissue lysate - whole (Muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 35 µg
Specification Muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Mar 06 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"