製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Myf5
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Orangutan
  • 免疫原

    Synthetic peptide within Human Myf5 aa 200-300. The exact sequence is proprietary.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Fetal Skeletal Muscle and Mouse E10 Whole Embryo tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 24699686
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

ターゲット情報

Anti-Myf5 antibody 画像

  • All lanes : Anti-Myf5 antibody (ab125301) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Human) Tissue Lysate - Fetal Tissue
    Lane 2 : Mouse Embryo E10 Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 46 kDa,52 kDa,98 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-Myf5 antibody (ab125301) 使用論文

This product has been referenced in:
  • Ren Y  et al. Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats. PLoS One 9:e93583 (2014). ICC/IF ; Goat . Read more (PubMed: 24699686) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (muscle, satellite cell)
Permeabilization
Yes - 0.5% TritonX100 in PBS
Specification
muscle, satellite cell
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 May 15 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (Brown adipose tissue)
Gel Running Conditions
Non-reduced Denaturing (4-12% Bis-Tris MOPS buffer, extended run (60 mins at 180v))
Loading amount
25 µg
Specification
Brown adipose tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
Username

Miss. Alice Pollard

Verified customer

投稿 May 09 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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