Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749)


  • 製品名
    Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric)
    Myeloperoxidase キット 製品一覧
  • サンプルの種類
    Cell culture supernatant, Tissue Extracts
  • アッセイタイプ
    Enzyme activity
  • 検出感度
    > 0.001 mU/well
  • 種交差性
    交差種: Mammal
  • 製品の概要

    Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749) provides a rapid, simple and reliable method to study MPO activity. MPO catalyzes the production of sodium hypochlorite (NaClO) from hydrogen peroxide (H2O2) and sodium chloride (NaCl). Subsequently, NaClO will react stoichiometrically with the free radical sensor Aminophenyl fluorescein (APF) to generate fluorescein, whose fluorescence can be detected at Ex/Em = 485/525 nm. This kit can be used to detect MPO activity as low as 0.5 µU per well. This kit is suitable for high throughput assay of MPO activity.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Myeloperoxidase (MPO) is a peroxidase enzyme (EC most abundantly present in neutrophil granulocytes. It is a green hemoprotein found in neutrophils and monocytes that catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates that play a role in the oxygen-dependent killing of tumor cells and microorganisms. Its heme pigment causes the green color in secretions rich in neutrophils, such as pus and some forms of mucus. Furthermore, it can oxidize tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent.

  • アプリケーション
    適用あり: Functional Studiesmore details



Our Abpromise guarantee covers the use of ab111749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.


  • a. Measurement of MPO activity in WBC lysate (0.1 μg) and MPO Positive Control (3 μL). b. MPO specific activity in WBC lysate.

  • Standard Curve using this kit protocol (Ex/Em = 485/525 nm)



This product has been referenced in:
  • You P  et al. Local level of TGF-ß1 determines the effectiveness of dexamethasone through regulating the balance of Treg/Th17 cells in TNBS-induced mouse colitis. Exp Ther Med 15:3639-3649 (2018). Read more (PubMed: 29545894) »
  • Roberts JS  et al. Opportunistic Pathogen Porphyromonas gingivalis Modulates Danger Signal ATP-Mediated Antibacterial NOX2 Pathways in Primary Epithelial Cells. Front Cell Infect Microbiol 7:291 (2017). Read more (PubMed: 28725637) »

See all 2 Publications for this product

レビューと Q&A

Thank you for contacting us.

I would recommend using fresh tissue samples.

Other peroxidases will not be able to produce the same intermediates required for this assay. Therefore it is specific for MPO.

I hope this infor...

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MPO activity of ovine lung tissue

Good Good 4/5 (Ease of Use)
Application: ELISA - MPO fluorometric assay
Samples: 10mg of homogenized lung tissue in 400uL of assay buffer
Loading amount: NEAT
Followed assay protocol to determine neutrophil infiltration in sheep lung tissue.
Standard curve: very high readings, out of range of neat samples - considered diluting standard to get within reference range
Positive control: was at the bottom end of the standard curve and used as a reference only to check the assay was working correctly

Ms. Gabriela Simonova

Verified customer

投稿 Sep 03 2014

The laboratory confirmed that the assay buffer will be compatible with a BCA assay, and this method of normalizing your samples' protein concentration would be appropriate in order to make activity level comparisons between your samples.

For peroxidase, fresh samples are the best. If needed the tissue can be quick-frozen in liquid nitrogen and stored at -80C. I would not advise making the lysate and then storing it for best results.

Thank you for your enquiry regarding ab111749.

We have always used with fresh samples only with this assay. We know of scientists who did store the homogenates in aliquots at -80°C for a short while before use but since we don’t our...

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The homogenized samples can be stored in aliquots at -80°C, w/o addition of any other components to the assay buffer. It would be preferable though to use fresh samples.
“V” is the volume of the sample you add to the wells in step ...

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This kit can be used with all mammalian samples including rats.

I have updated our datasheet accordingly.

This is an enzymatic activity assay. Enzymes need some ions/cofactors to function and EDTA being a chelator will chelate these co-factors (specifically Mg++ and Ca++), leading to the enzyme becoming non-functional.

Thank you for your inquiry and your patience with this reply.

I heard back from the supplying laboratory that the concentration of the positive control MPO does not vary from lot to lot. But unfortunately, they could not disclose the concent...

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For this assay kit, the client can either use a black opaque bottom plate or, for even more efficiency, use a white opaque bottom plate. One would not use a clear transparent plate.

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