製品の概要

  • 製品名Anti-Myeloperoxidase antibody [EPR4792]
    Myeloperoxidase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4792] to Myeloperoxidase
  • 特異性ab109116 recognizes Myeloperoxidase (85-90 kDa) and its light chain (15 kDa).
  • アプリケーション適用あり: ICC/IF, WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human Myeloperoxidase.

  • ポジティブ・コントロール
    • WB: HL-60 cell lysate IHC-P: Human colon, lung and spleen tissues
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 24691127
WB 1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
ICC 1/250 - 1/500.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
    • 関連疾患Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
    • 配列類似性Belongs to the peroxidase family. XPO subfamily.
    • 細胞内局在Lysosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 84 kDa myeloperoxidase antibody
      • 89 kDa myeloperoxidase antibody
      • EC 1.11.1.7 antibody
      • EC1.11.2.2 antibody
      • fj80f04 antibody
      • MPO antibody
      • mpx antibody
      • myeloid-specific peroxidase antibody
      • Myeloperoxidase antibody
      • Myeloperoxidase heavy chain antibody
      • Myeloperoxidase light chain antibody
      • PERM_HUMAN antibody
      • wu:fj80f04 antibody
      see all

    Anti-Myeloperoxidase antibody [EPR4792] 画像

    • Anti-Myeloperoxidase antibody [EPR4792] (ab109116) at 1/1000 dilution + HL-60 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 84 kDa
      Observed band size : 85-90 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 15 kDa (possible cleavage fragment).
    • ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human colon tissue by Immunohistochemistry.
    • ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human lung tissue by Immunohistochemistry.
    • ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human spleen tissue by Immunohistochemistry.
    • ab109116 showing positive staining in Normal liver tissue.

    • ab109116 showing positive staining in Bone marrow tissue.

    • ab109116 showing negative staining in Skeletal muscle tissue.

    Anti-Myeloperoxidase antibody [EPR4792] (ab109116) 使用論文

    This product has been referenced in:
    • Baek JH  et al. Extracellular Hb enhances cardiac toxicity in endotoxemic guinea pigs: protective role of haptoglobin. Toxins (Basel) 6:1244-59 (2014). WB, ICC/IF ; Guinea pig . Read more (PubMed: 24691127) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab109116.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"