製品の概要

  • 製品名Anti-Myeloperoxidase antibody
    Myeloperoxidase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Myeloperoxidase
  • アプリケーション適用あり: IHC-P, IHC-Fr, ICC/IF, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig, Monkey
  • 免疫原

    Myeloperoxidase isolated from human granulocytes.

  • ポジティブ・コントロール
    • Tonsil

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/25 - 1/50.
IHC-Fr 1/100.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

ターゲット情報

  • 機能Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • 関連疾患Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • 配列類似性Belongs to the peroxidase family. XPO subfamily.
  • 細胞内局在Lysosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 84 kDa myeloperoxidase antibody
    • 89 kDa myeloperoxidase antibody
    • EC 1.11.1.7 antibody
    • EC1.11.2.2 antibody
    • fj80f04 antibody
    • MPO antibody
    • mpx antibody
    • myeloid-specific peroxidase antibody
    • Myeloperoxidase antibody
    • Myeloperoxidase heavy chain antibody
    • Myeloperoxidase light chain antibody
    • PERM_HUMAN antibody
    • wu:fj80f04 antibody
    see all

Anti-Myeloperoxidase antibody 画像

  • ab9535 staining Myeloperoxidase in paraffin-embedded rat tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded Immunohistochemistry). Tissue was fixed with formaldehyde and blocked with 20% serum for 1 hour at 24°C; antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were incubated with primary antibody (1/50 in milken) for 1 hour ate 24°C. A Biotin-conjugated goat anti rabbt polyclonal (1/500) was used as secondary antibody.

    See Abreview

  • Ab9535 staining of multinucleated cell (Blue) in human blood smear. Antibody was used at 1/200 for 1 hour at 21°C.

    See Abreview

  • Immunohistochemistry (Frozen sections) of Mouse stomach tissue labelling Myeloperoxidase with ab9535 at 5%.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human B Cell Lymphoma tissue, staining Myeloperoxidase with ab9535.

Anti-Myeloperoxidase antibody (ab9535) 使用論文

This product has been referenced in:
  • Ambade A  et al. Adult mouse model of early hepatocellular carcinoma promoted by alcoholic liver disease. World J Gastroenterol 22:4091-108 (2016). IHC-P ; Mouse . Read more (PubMed: 27122661) »
  • Ueno K  et al. Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblasts. Sci Rep 6:28538 (2016). IHC-P ; Mouse . Read more (PubMed: 27329845) »

See all 24 Publications for this product

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0
Permeabilization No
Specification Lung
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative Formaldehyde
Username

Mr. Simon Cleary

Verified customer

投稿 May 09 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Normal Spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH9, Tris/EDTA based
Permeabilization No
Specification Normal Spleen
Blocking step serum Free Protein Block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative 10% NBF
Username

Ms. Premier Laboratory

Verified customer

投稿 Apr 11 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (stomach)
Permeabilization No
Specification stomach
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Mar 10 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Bone)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization No
Specification Bone
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered formalin
Username

Abcam user community

Verified customer

投稿 Feb 17 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (liver treated with LPS)
Permeabilization No
Specification liver treated with LPS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 14 2015

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (LPS-treated lung)
Permeabilization No
Specification LPS-treated lung
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative Formaldehyde
Username

Mr. Simon Cleary

Verified customer

投稿 Dec 03 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Blood smear)
Permeabilization No
Specification Blood smear
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative Formaldehyde
Username

Mr. Simon Cleary

Verified customer

投稿 Dec 01 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (skin tumor)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization No
Specification skin tumor
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered formalin
Username

Abcam user community

Verified customer

投稿 Nov 10 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
Permeabilization No
Specification lung
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered formalin
Username

Abcam user community

Verified customer

投稿 Aug 17 2015

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"