製品の概要

  • 製品名
    Anti-Myeloperoxidase antibody
    Myeloperoxidase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Myeloperoxidase
  • 特異性
    ab45977 has been batch tested using human lysates/tissues only. Some customers have successfully used ab45977 with rat. Please contact Abcam Scientific Support for more information.
  • アプリケーション
    適用あり: IHC-Fr, ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human, Common marmoset
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Myeloperoxidase.

    (Peptide available as ab45976.)

  • ポジティブ・コントロール
    • WB: HL60 whole cell lysate. IHC-P: Human tonsil tissue sections. ICC-IF. HL60 cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 - 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).Can be blocked with Myeloperoxidase peptide (ab45976).
IHC-P Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • 関連疾患
    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • 配列類似性
    Belongs to the peroxidase family. XPO subfamily.
  • 細胞内局在
    Lysosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 84 kDa myeloperoxidase antibody
    • 89 kDa myeloperoxidase antibody
    • EC 1.11.1.7 antibody
    • EC1.11.2.2 antibody
    • fj80f04 antibody
    • MPO antibody
    • mpx antibody
    • myeloid-specific peroxidase antibody
    • Myeloperoxidase antibody
    • Myeloperoxidase heavy chain antibody
    • Myeloperoxidase light chain antibody
    • PERM_HUMAN antibody
    • wu:fj80f04 antibody
    see all

画像

  • ab45977 stained in HL60 cells. Cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab45977 at 5µg/ml and ab72791 (Mouse monoclonal to alpha Tubulin - Loading Control) at 1ug/ml overnight at +4°C. The secondary antibodies used were ab150081 used at 1 ug/ml (colored green) and ab150116 (pseudo-colored red) used at 2ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • ab45977 (1/50) staining Myeloperoxidase in paraffin-embbeded human spleen tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block and 10 minutes/protein block). For further experimental details please refer to abreview.

    See Abreview

  • Anti-Myeloperoxidase antibody (ab45977) at 1 µg/ml + HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 84 kDa


    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab45977 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

  • ab23106 staining p53 in Mouse bone marrow cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 5% Goat serum for 2 hours at 25°C. The primary antibody was diluted 1/500 in PBS and incubated with the sample for 9 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/500.
    Nuclei were counterstained blue with DAPI.

    See Abreview

  • Immunohistochemistical detection of Myeloperoxidase using antibody ab45977 on formaldehyde-fixed paraffin embedded Marmoset spleen sections.  Antigen retrieval step: heat mediated in citric acidpH6 HIER. Permeabilization: No Blocking step: 1% BSA for 10 mins at room temperature. Primary antibody diluted at 1/600 and incubated for 2 hours in TBS/BSA/azide. Secondary antibody: anti Rabbit IgG conjugated to biotin (1/200). Submitted image shows clear scattered cytoplasmic and (possibly secreted) positivity within the red pulp. Also, the blood vessel extending from upper middle to lower left of the image, shows clear, though lighter, granular positivity within the cytoplasm of 5 cells. Taken together, this is the pattern of positivity that I would expect.

    See Abreview

  • IHC image of 45977 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45977, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Anti-Myeloperoxidase antibody (ab45977) at 1 µg/ml + HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 84 kDa
    Observed band size : 84 kDa
    Additional bands at : 12 kDa,35 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

    The mature myeloperoxidase protein is a tetramer of two heavy chains (60 kDa) and two light chains (12 kDa).  Our immunogen sequence is within the myeloperoxidase light chain.  In HL60 cells, ab45977 detects bands at approximately 84-kDa, corresponding to the expected MW of full-length Myeloperoxidase protein, and at 12-kDa, corresponding to the expected MW of myeloperoxidase light chain.   

参考文献

This product has been referenced in:
  • Cao S  et al. Hydrogen sulfide attenuates brain edema in early brain injury after subarachnoid hemorrhage in rats: Possible involvement of MMP-9 induced blood-brain barrier disruption and AQP4 expression. Neurosci Lett 621:88-97 (2016). IHC ; Rat . Read more (PubMed: 27080433) »
  • Shi Y  et al. Rapid endothelial cytoskeletal reorganization enables early blood-brain barrier disruption and long-term ischaemic reperfusion brain injury. Nat Commun 7:10523 (2016). IHC-P ; Mouse . Read more (PubMed: 26813496) »

See all 33 Publications for this product

レビューと Q&A

Thank you for contacting us.

As discussed please send us the purchase order number. We will then check what went wrong with these antibodies.

Thanks!

Thank you for your reply.

Please let me know if the antibody worked well with human samples.

If not, please let me know the details of your experiment and what the problem was. In that case, I'd be happy to offer you a replacement,...

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Spleen)
Specification
Spleen
Fixative
Acetone
Permeabilization
Yes - 0.1%PBS Tween
Blocking step
BSA as blocking agent for 1 hour(s) and 10 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Aronaghy

Verified customer

投稿 Oct 24 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat cell lysate)
Loading amount
30 µg
Specification
Jurkat cell lysate
Gel Running Conditions
Reduced Denaturing (8% Gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

投稿 Oct 16 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer, pH6
Permeabilization
No
Specification
spleen
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 13 2012

Thank you for contacting us.

These products are sold in liquid form and should not require any reconstitution. When using in your particular assay please dilute in the appropriate buffer for that assay.

I hope this information...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jul 05 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6.0
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 05 2012

1-10 of 27 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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